抗CD15抗体(Anti-CD15, Human/Mouse, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	F9 mouse teratocarcinoma stem cells
Source	Monoclonal Mouse IgM Clone # MC-480
Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Specificity	Detects human and mouse SSEA-1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	D3 mouse embryonic stem cell line
Immunoprecipitation	Ballou, B. et al. (1979) Science 206:844.
CyTOF-reported	Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry
	SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

SSEA-1

background_content

Background: SSEA-1 Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

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Citations

1. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
   Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
   Stem Cell Res, 2017;20(0):105-114.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
   Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
   Oxid Med Cell Longev, 2017;2017(0):4745252.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation
   Authors: M Klimczak, P Czerwi?ska, S Mazurek, B Soza?ska, P Biecek, A Mackiewicz, M Wiznerowic
   Stem Cell Res, 2017;23(0):163-172.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
   Authors: Boo, Kyungjin, Bhin, Jinhyuk, Jeon, Yoon, Kim, Joomyung, Shin, Hi-Jai R, Park, Jong-Eun, Kim, Kyeongky, Kim, Chang Ro, Jang, Hyonchol, Kim, In-Hoo, Kim, V Narry, Hwang, Daehee, Lee, Ho, Baek, Sung Hee
   Nat Commun, 2015;6(0):6810.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Not Specified


7. Pluripotent stem cells derived from mouse and human white mature adipocytes.
   Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
   Stem Cells Transl Med, 2014;3(2):161-71.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
   Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
   Stem Cells, 2014;32(10):2626-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
   BMC Vet Res, 2012;8(0):150.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


10. Aire regulates the expression of differentiation-associated genes and self-renewal of embryonic stem cells.
    Authors: Gu B, Zhang J, Chen Q, Tao B, Wang W, Zhou Y, Chen L, Liu Y, Zhang M
    Biochem. Biophys. Res. Commun., 2010;394(2):418-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

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