抗Vimentin抗体(Anti-Vimentin, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human VimentinSer2-Glu466Accession # P08670
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Vimentin in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Knockout Validated	Vimentin is specifically detected in K562 human chronic myelogenous leukemia cell line but is not detectable in Vimentin knockout K562 cell line.

Western Blot
	Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 µg/mL Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunocytochemistry
	Vimentin in HeLa Human Cell Line. Vimentin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Knockout Validated
	Vimentin Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Vimentin was detected in immersion fixed K562 human chronic myelogenous leukemia cell line but is not detected in Vimentin knockout (KO) K562 Human Cell Line cell line using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Simple Western
	Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

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Related Product & Information

Background

Vimentin

background_content

Background: Vimentin Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1‑3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97‑98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10‑12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9‑11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

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Citations

1. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
   Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
   Respir. Res., 2017;18(1):82.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


2. Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
   Authors: Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
   PLoS ONE, 2016;11(1):e0147155.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Not Specified


3. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
   PLoS ONE, 2016;11(9):e0162221.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery
   Authors: David G Gorenstein
   BioTechniques, 2016;61(5):249-259.
   Species: Human
   Sample Type: Whole Cells
   Application: Binding Assay


5. The mosaic of "seronegative" antiphospholipid syndrome.
   Authors: Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
   J Immunol Res, 2014;2014(0):389601.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


6. Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
   Authors: Teo, Catherin, Chu, Justin J
   J Virol, 2014;88(4):1897-913.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
   Authors: Windus L, Kiss D, Glover T, Avery V
   Exp Cell Res, 2012;318(19):2507-19.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
   Authors: Lai D, Ma L, Wang F
   Int. J. Oncol., 2012;41(2):541-50.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Angiotensin II Contributes to Renal Fibrosis Independently of Notch Pathway Activation.
   Authors: Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
   PLoS ONE, 2012;7(7):e40490.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


10. Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
    Authors: Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
    Blood, 2010;116(16):2960-7.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development

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