抗Human M-CSF R抗体(Anti-Human M-CSF R antibody)
掲載日情報：2018/11/26 現在Webページ番号：30459
Human M-CSF Rに対する抗体(Anti-Human M-CSF R )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月01日 11時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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Anti-Human M-CSF R MAb (Clone 61701) |
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Anti-Human M-CSF R MAb (Clone 61701) |
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0 | |||
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Anti-Human M-CSF R MAb (Clone 61701) |
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0 | |||
[在庫・価格 ：2024年05月01日 11時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human M-CSF R MAb (Clone 61701)
文献数： 0
- 商品コード：MAB3291-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human M-CSF R MAb (Clone 61701)
文献数： 0
- 商品コード：MAB3291-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human M-CSF R MAb (Clone 61701)
文献数： 0
- 商品コード：MAB3291-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115Ile20-Glu512 (Pro54Ala)Accession # P07333.2 |
| Source | Monoclonal Mouse IgG1 Clone # 61701 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Neutralization | Measured by its ability to neutralize M-CSF R/CD115-mediated inhibition of proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND50) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human M-CSF R/CD115 Fc Chimera and 1 ng/mL Recombinant Human M-CSF. | |
| Western Blot | |
|---|---|
 | Detection of Human M-CSF R/CD115 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Neutralization | |
 | M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by HumanM-CSF R/CD115 Antibody. Recombinant HumanM-CSF R/CD115 Fc Chimera (Catalog # 329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant HumanM-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND50 is typically 4-16 ng/mL. |
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Related Product & Information
| Long Name | Macrophage Colony Stimulating Factor Receptor |
|---|---|
| Entrez Gene IDs | 1436 (Human) |
| Background | M-CSF R/CD115 |
| background_content | Background: M-CSF R/CD115 M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- IL-34 Upregulated Th17 Production through Increased IL-6 Expression by Rheumatoid Fibroblast-Like Synoviocytes
Authors: B Wang, Z Ma, M Wang, X Sun, Y Tang, M Li, Y Zhang, F Li, X Li
Mediators Inflamm., 2017;2017(0):1567120.
Species: Human
Sample Type: Whole Cells
Application: Neut - Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
PLoS ONE, 2016;11(11):e0167324.
Species: Human
Sample Type: Whole Cells
Application: Neut - Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFbeta in claudin-low breast tumor cells.
Authors: Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis J
Oncogene, 2015;34(21):2721-31.
Species: Human
Sample Type: Whole Cells
Application: Neut - Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer.
Authors: Forget M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, Moldovan L, Mo X, Evans R, Marsh C, Eubank T
PLoS ONE, 2014;9(6):e98623. - Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma.
Authors: Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S
Nat. Med., 2010;16(5):571-9.
Species: Human
Sample Type: Cell Lysates
Application: IP
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