抗Human CD163 Allophycocyanin抗体(Anti-Human CD163 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CD163Gly46-Ser1050Accession # Q86VB7
Source	Monoclonal Mouse IgG1 Clone # 215927
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD163 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CD163 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD163 APC‑conjugated Monoclonal Antibody (Catalog # FAB1607A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

CD163

background_content

Background: CD163 CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

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Citations

1. Ipilimumab-dependent cell-mediated cytotoxicity of regulatory T cells ex vivo by nonclassical monocytes in melanoma patients.
   Authors: Romano E, Kusio-Kobialka M, Foukas P, Baumgaertner P, Meyer C, Ballabeni P, Michielin O, Weide B, Romero P, Speiser D
   Proc Natl Acad Sci U S A, 2015;112(19):6140-5.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
   Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor, Walther, Michael
   J Immunol, 2012;189(11):5336-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
   Authors: Johnson TR, McLellan JS, Graham BS
   J. Virol., 2012;86(3):1339-47.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
   Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
   J. Thromb. Haemost., 2011;9(5):1056-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Modulation of Decidual Macrophage Polarization by Macrophage Colony-Stimulating Factor Derived from First-Trimester Decidual Cells: Implication in Preeclampsia.
   Authors: Li M, Piao L, Chen C, Wu X, Yeh C, Masch R, Chang C, Huang S
   Am J Pathol, 0;186(5):1258-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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