抗Human CD25/IL-2 R alpha Phycoerythrin抗体(Anti-Human CD25/IL-2 R alpha Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213Accession # P01589
Source	Monoclonal Mouse IgG2A Clone # 24212
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of Human CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog #240-B) for 2 days were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1020P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Interleukin 2 Receptor alpha
Background	CD25/IL-2 R alpha
background_content	Background: CD25/IL-2 R alpha Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

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Citations

1. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
   Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
   J Immunol, 2014;192(1):123-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
   Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
   2011;6(5):e19988.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
   Authors: Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M
   Eur. J. Immunol., 2010;40(1):197-203.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
   Authors: Ni Z, Walcheck B
   Clin. Immunol., 2009;133(2):257-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
   Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
   FEBS Lett., 2007;581(7):1317-22.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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