抗SOST抗体(Anti-SOST, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse SOST/SclerostinGln24-Tyr211Accession # NP_077769
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects SOST/Sclerostin in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human SOST is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse SOST/Sclerostin (Catalog # 1589-ST)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse SOST/Sclerostin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

SOST/Sclerostin

background_content

Background: SOST/Sclerostin SOST, also known as Sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1‑3).

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Citations

1. Differential involvement of Wnt signaling in Bmp regulation of cancellous versus periosteal bone growth
   Authors: G He, Y Shi, J Lim, T Bellido, J Ni, F Long
   Bone Res, 2017;5(0):17016.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Loss of Gsalpha in the Postnatal Skeleton Leads to Low Bone Mass and a Blunted Response to Anabolic Parathyroid Hormone Therapy.
   Authors: Sinha P, Aarnisalo P, Chubb R, Poulton I, Guo J, Nachtrab G, Kimura T, Swami S, Saeed H, Chen M, Weinstein L, Schipani E, Sims N, Kronenberg H, Wu J
   J Biol Chem, 2016;291(4):1631-42.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs
   Authors: Z Liu, T Yamamoto, T Hasegawa, H Hongo, K Tsuboi, E Tsuchiya, M Haraguchi, M Abe, PH Freitas, A Kudo, K Oda, M Li, N Amizuka
   Biomed Res, 2016;37(2):141-51.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.
   Authors: Spatz J, Wein M, Gooi J, Qu Y, Garr J, Liu S, Barry K, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg H, Babij P, Pajevic P
   J Biol Chem, 2015;290(27):16744-58.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC OCT-embedded


5. Gsalpha enhances commitment of mesenchymal progenitors to the osteoblast lineage but restrains osteoblast differentiation in mice.
   Authors: Wu JY, Aarnisalo P, Bastepe M, Sinha P, Fulzele K, Selig MK, Chen M, Poulton IJ, Purton LE, Sims NA, Weinstein LS, Kronenberg HM
   J. Clin. Invest., 2011;121(9):3492-504.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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