抗Brachyury抗体(Anti-Brachyury, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human BrachyurySer2-Glu202Accession # O15178
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) TBX-6, rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	Brachyury/DNA immunocomplexes were detected in sheared chromatin from fixed BG01V Human embryonic stem cells using standard PCR for the VEGF promoter sequence. Use 5 μg of antibody per 5x106 cells.
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Recombinant Human Brachyury by Western Blot. Western blot shows 10 ng of Recombinant Human Brachyury and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
	Brachyury in BG01V Human Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Brachyury in Differentiated Human Embryonic Stem Cells. Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Brachyury in Embryonic Mouse Notochord. Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Chromatin Immunoprecipitation (ChIP)
	Detection of Brachyury-regulated Genes by Chromatin Immunoprecipitation. Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.

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Related Product & Information

Entrez Gene IDs	6862 (Human)
Background	Brachyury
background_content	Background: Brachyury Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

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Citations

1. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
   Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
   Cell Stem Cell, 2018;22(4):543-558.e12.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
   Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
   Cell Death Dis, 2018;9(1):6.
   Species: Human
   Sample Type: Embryoid Bodies
   Application: ICC


3. A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos
   Authors: M Oginuma, P Moncuquet, F Xiong, E Karoly, J Chal, K Guevorkian, O Pourquié
   Dev. Cell, 2017;40(4):342-353.e10.
   Species: Chicken
   Sample Type: Whole Tissue
   Application: IHC-F


4. Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female
   Authors: S Martins, M Bohndorf, F Schröter, F Assar, W Wruck, K Sleegers, C Van Broeck, J Adjaye
   Stem Cell Res, 2017;26(0):76-79.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency
   Authors: CY Yu, TC Li, YY Wu, CH Yeh, W Chiang, CY Chuang, HC Kuo
   Nat Commun, 2017;8(1):1149.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
   Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
   Stem Cell Reports, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Guided self-organization and cortical plate formation in human brain organoids
   Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
   Nat. Biotechnol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening
   Authors: J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu
   Sci Rep, 2017;7(1):8491.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells
   Authors: Joan Massagué
   Cell Stem Cell, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells
    PLoS ONE, 2016;11(7):e0159246.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Fresh

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