抗IL-23抗体(Anti-IL-23, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑23 p19Met1-Ala196Accession # Q9EQ14
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑12/23 p40 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑23 (Catalog # 1887-ML)
Neutralization	Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND50) is typically 0.07-0.35 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.

Neutralization

IL‑17 secretion Induced by IL‑23 and Neutralization by Mouse IL‑23 Antibody. In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402‑ML), Recombinant Mouse IL‑23 (Catalog # 1887-ML) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Mouse IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND50 is typically 0.07-0.35 µg/mL.

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Related Product & Information

Long Name	Interleukin 23
Entrez Gene IDs	51561 (Human); 83430 (Mouse)
Background	IL-23
background_content	Background: IL-23 Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

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Citations

1. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation
   Authors: E Marks, C Naudin, G Nolan, BJ Goggins, G Burns, SW Mateer, JK Latimore, K Minahan, M Plank, PS Foster, R Callister, M Veysey, MM Walker, NJ Talley, G Radford-Sm, S Keely
   Mucosal Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


2. IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury
   Authors: M Geha, MG Tsokos, RE Bosse, T Sannikova, Y Iwakura, JJ Dalle Lucc, R De Waal Ma, GC Tsokos
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. Dendritic Cells in Irradiated Mice Trigger the Functional Plasticity and Antitumor Activity of Adoptively Transferred Tc17 Cells via IL12 Signaling.
   Authors: Bowers J, Nelson M, Kundimi S, Bailey S, Huff L, Schwartz K, Cole D, Rubinstein M, Paulos C
   Clin Cancer Res, 2015;21(11):2546-57.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


4. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
   Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
   Infect. Immun., 2012;80(1):410-7.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. RORgammat+ innate lymphoid cells regulate intestinal homeostasis by integrating negative signals from the symbiotic microbiota.
   Authors: Sawa S, Lochner M, Satoh-Takayama N, Dulauroy S, Berard M, Kleinschek M, Cua D, Di Santo JP, Eberl G
   Nat. Immunol., 2011;12(4):320-6.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
   Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
   J. Immunol., 2011;186(2):869-77.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
   Authors: El-Behi M, Ciric B, Dai H
   Nat. Immunol., 2011;12(6):568-75.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
   Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
   J. Exp. Med., 2011;208(3):491-503.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


9. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
   Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
   Nat. Med., 2010;16(2):191-7.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


10. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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