抗CD16抗体(Anti-CD16, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIB (R&D Systems, Catalog # 1597-FC)Thr20-Gln208Accession # O75015
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant human Fc gamma  RIIA is observed and less than 2% cross-reactivity with recombinant mouse CD16 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Fc gamma  RIIIB/CD16b (Catalog # 1597-FC)
Blockade of Receptor-ligand Interaction	In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 5 µg/mL of human IgG to immobilized Recombinant Human Fc gamma  RIIIB/CD16b (Catalog # 1597-FC) coated at 5 µg/mL (100 µL/well). At 50 µg/mL, this antibody will block >90% of the binding.

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Related Product & Information

Background

Fc gamma RIII (CD16)

background_content

Background: Fc gamma RIII (CD16) Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells, and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.

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Citations

1. Microvesicles released by apoptotic human neutrophils suppress proliferation and IL-2/ IL-2 receptor expression of resting T helper cells
   Authors: G Shen, S Krienke, P Schiller, A Nie beta en, S Neu, V Eckstein, M Schiller, HM Lorenz, LO Tykocinski
   Eur. J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Multivalent Fc?-receptor engagement by a hexameric Fc-fusion protein triggers Fc?-receptor internalisation and modulation of Fc?-receptor functions
   Authors: OS Qureshi, TF Rowley, F Junker, SJ Peters, S Crilly, J Compson, A Eddleston, H Björkelund, K Greenslade, M Parkinson, NL Davies, R Griffin, TL Pither, K Cain, L Christodou, L Staelens, E Ward, J Tibbitts, A Kiessling, B Smith, FR Brennan, M Malmqvist, F Fallah-Ara, DP Humphreys
   Sci Rep, 2017;7(1):17049.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset.
   Authors: Chauhan A, Chen C, Moore T, DiPaolo R
   J Biol Chem, 2015;290(8):5127-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells.
   Authors: Chauhan AK, Moore TL
   Clin. Exp. Immunol., 2012;167(2):235-45.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
   Authors: Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
   J. Clin. Invest., 2010;120(12):4236-50.
   Species: Human
   Sample Type: Serum
   Application: WB

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