抗Porcine CXCL8/IL-8抗体(Anti-Porcine CXCL8/IL-8 antibody)
掲載日情報：2021/01/28 現在Webページ番号：30240
Porcine CXCL8/IL-8に対する抗体(Anti-Porcine CXCL8/IL-8 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-Porcine CXCL8/IL-8 MAb (Clone 105105) |
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Anti-Porcine CXCL8/IL-8 MAb (Clone 105105) |
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Anti-Porcine CXCL8/IL-8 MAb (Clone 105105) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-Porcine CXCL8/IL-8 MAb (Clone 105105)
文献数： 1
- 商品コード：MAB5351-100
- メーカー：RSD
- 包装：100μg
- 価格：¥97,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：3-10C クローン：105105 Genbank No： 3576 Protein Accession No： CAA43461 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Porcine CXCL8/IL-8 MAb (Clone 105105)
文献数： 1
- 商品コード：MAB5351-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Porcine CXCL8/IL-8 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF535，スタンダードとして#535-IN-025を用いる。 別名：3-10C クローン：105105 Genbank No： 3576 Protein Accession No： CAA43461 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Porcine CXCL8/IL-8 MAb (Clone 105105)
文献数： 1
- 商品コード：MAB5351-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：3-10C クローン：105105 Genbank No： 3576 Protein Accession No： CAA43461 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Porcine |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant porcine IL-8/CXCL8Ala26-Gln104Accession # CAA43461 |
| Source | Monoclonal Mouse IgG1 Clone # 105105 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects porcine IL-8/CXCL8 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, rhCXCL13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, rmCXCL13, recombinant rat (rr) CXCL1, rrCXCL3/CINC2 alpha, or rr3/CINC-2 beta is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) under non-reducing conditions only |
| Porcine IL-8/CXCL8 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Porcine IL-8/CXCL8 Antibody (Catalog #MAB5351 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Porcine IL-8/CXCL8 Biotinylated Antibody (Catalog #BAF535 ) |
| ELISA Standard | Recombinant Porcine IL-8/CXCL8 Protein (Catalog #535-IN ) | |
| Neutralization | Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 50 ng/mL Recombinant Porcine IL-8/CXCL8. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization CXCL8/IL-8 Antibody (105105) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CXCL8_MAB5351_Block_Neutralize_9231.jpg) click image to view larger | Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody.Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-8/CXCL8 | Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro. |
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References
| Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271. | |
| Long Name: | Interleukin 8 |
| Entrez Gene IDs: | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
| Alternate Names: | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 |
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Related Research Areas
| Adipocytokines | ||||||||||
| Alpha Granule Soluble Molecules | ||||||||||
| Cancer Biomarkers | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| CXC Chemokines and Receptors | ||||||||||
| Diabetes | ||||||||||
| HIF Transcription Factors | ||||||||||
| Infectious Diseases | ||||||||||
| Inflammatory Mediators | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Megakaryocytes | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
| Neuroinflammation | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Porcine | Anonymous | 11/08/2017 | Image Details |
 ELISA: Porcine IL-8/CXCL8 Antibody [MAB5351]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Porcine |
| Other Experimental Details | The antibody MAB5351 was used as the capture antibody in a sandwich ELISA for Porcine IL-8 along with the detection antibody, AF535. The calibrator for the assay was recombinant porcine IL-8, 535-IN. It was sensitive and specific. |
| Neutralization | |
|---|---|
 | Chemotaxis Induced byIL-8/CXCL8 and Neutralization by Porcine IL-8/CXCL8 Antibody. Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant PorcineIL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND50 is typically 1-6 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 8 |
|---|---|
| Entrez Gene IDs | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
| Background | IL-8/CXCL8 |
| background_content | Background: IL-8/CXCL8 Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro‑inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. Recently, IL-8 was reported to be angiogenic both in vivo and in vitro. |
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Citations
- Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
Infect. Immun., 2017;85(4):.
Species: Porcine
Sample Type: Serum
Application: ELISA Capture - The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
Intensive Care Med, 2007;33(6):1085-93.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
Cytometry A, 2006;69(5):391-5.
Species: Porcine
Sample Type: Serum
Application: Luminex Development - Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
Anesthesiology, 2004;100(4):861-70.
Species: Porcine
Sample Type: Plasma
Application: ELISA Development
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