抗Human Endoglin/CD105 Fluorescein抗体(Anti-Human Endoglin/CD105 Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105Glu26-Gly586Accession # Q5T9B9
Source	Monoclonal Mouse IgG1 Clone # 166707
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Endoglin/CD105.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB10971F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Endoglin/CD105

background_content

Background: Endoglin/CD105 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

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Citations

1. Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
   Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
   BMC Cell Biol., 2017;18(1):21.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
   Authors: SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn
   Stem Cell Res Ther, 2017;8(1):140.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Adenosine triphosphate prevents serum deprivation-induced apoptosis in human mesenchymal stem cells via activation of the MAPK signaling pathways.
   Authors: Berlier J, Rigutto S, Dalla Valle A, Lechanteur J, Soyfoo M, Gangji V, Rasschaert J
   Stem Cells, 2015;33(1):211-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Proinflammatory Mediators Enhance the Osteogenesis of Human Mesenchymal Stem Cells after Lineage Commitment.
   Authors: Croes M, Oner F, Kruyt M, Blokhuis T, Bastian O, Dhert W, Alblas J
   PLoS ONE, 2015;10(7):e0132781.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.
   Authors: Narcisi R, Cleary M, Brama P, Hoogduijn M, Tuysuz N, ten Berge D, van Osch G
   Stem Cell Reports, 2015;4(3):459-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
   Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
   Stem Cells Dev, 2014;23(5):541-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Human dermis harbors distinct mesenchymal stromal cell subsets.
   Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
   J. Invest. Dermatol., 2012;132(3):563-74.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
   Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
   PLoS ONE, 2012;7(10):e47187.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro.
   Authors: Zhang Y, Fisher N, Newey SE
   Stem Cells Dev., 2009;18(2):359-75.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Donor-derived mesenchymal stem cells suppress alloreactivity of kidney transplant patients.
    Authors: Crop MJ, Baan CC, Korevaar SS, Ijzermans JN, Alwayn IP, Weimar W, Hoogduijn MJ
    Transplantation, 2009;87(6):896-906.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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