抗IL-18/IL-1F4抗体(Anti-IL-18/IL-1F4, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat IL‑18/IL-1F4His37-Ser194Accession # P97636.1
Source	Monoclonal Mouse IgG1 Clone # 69604
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects rat IL‑18/IL-1F4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑18 and no cross-reactivity with recombinant human IL-18, recombinant porcine IL-18, recombinant rat (rr) IL-1 alpha, or rrIL-1 beta.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Rat IL‑18/IL‑1F4 (Catalog # 521-RL)

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Related Product & Information

Background

IL-18/IL-1F4

background_content

Background: IL-18/IL-1F4 Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rat IL‑18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60% - 64% aa sequence identity with human, canine, feline, porcine, and rhesus IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).

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Citations

1. Mitochondria Protection after Acute Ischemia Prevents Prolonged Upregulation of IL-1? and IL-18 and Arrests CKD
   Authors: HH Szeto, S Liu, Y Soong, SV Seshan, L Cohen-Goul, V Manichev, LC Feldman, T Gustafsson
   J. Am. Soc. Nephrol., 2017;28(5):1437-1449.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


2. Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade.
   Authors: Guo W, Miyoshi K, Dubner R, Gu M, Li M, Liu J, Yang J, Zou S, Ren K, Noguchi K, Wei F
   Mol Pain, 2014;10(0):35.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


3. Proteasome inhibitors prevent caspase-1-mediated disease in rodents challenged with anthrax lethal toxin.
   Authors: Muehlbauer SM, Lima H, Goldman DL, Jacobson LS, Rivera J, Goldberg MF, Palladino MA, Casadevall A, Brojatsch J
   Am. J. Pathol., 2010;177(2):735-43.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


4. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
   Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
   Arthritis Rheum., 2006;54(8):2402-14.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: ELISA Development

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