抗M-CSF抗体(Anti-M-CSF, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse M-CSFLys33-Glu262 (predicted)Accession # P07141
Source	Monoclonal Rat IgG2B Clone # 131614
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse M-CSF (Catalog # 416-ML)
Neutralization	Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line. Halenbeck, R. et al. (1989) Biotechnology 7:710. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.

Neutralization

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M‑CSF Monoclonal Antibody (Catalog # MAB4161). The ND50 is typically 3-12 µg/mL.

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Related Product & Information

Long Name	Macrophage Colony Stimulating Factor
Entrez Gene IDs	1435 (Human); 12977 (Mouse)
Background	M-CSF
background_content	Background: M-CSF M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

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Citations

1. An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system
   Authors: K Choi, K Komurov, JS Fletcher, E Jousma, JA Cancelas, J Wu, N Ratner
   Sci Rep, 2017;7(0):43315.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
   Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H
   J Leukoc Biol, 2014;95(1):19-31.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival.
   Authors: Luo, Jian, Elwood, Fiona, Britschgi, Markus, Villeda, Saul, Zhang, Hui, Ding, Zhaoqing, Zhu, Liyin, Alabsi, Haitham, Getachew, Ruth, Narasimhan, Ramya, Wabl, Rafael, Fainberg, Nina, James, Michelle, Wong, Gordon, Relton, Jane, Gambhir, Sanjiv S, Pollard, Jeffrey, Wyss-Coray, Tony
   J Exp Med, 2013;210(1):157-72.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


4. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
   Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
   Blood, 2007;109(9):3706-12.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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