抗Mouse CD4抗体(Anti-Mouse CD4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse CTL clone V4
Source	Monoclonal Rat IgG2B Clone # GK1.5
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	Mouse splenocytes
Immunohistochemistry	1-25 µg/mL	See below
Immunoprecipitation	Dialynas, D.P. et al. (1983) J. Immunol. 131:2445.
Cell depletion	Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Martin, P. et al. (2000) Blood 96:2511.
Costimulation of T Cells	Bosselut, R. et al. (1999) J. Exp. Med. 190:1517.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Inhibition of T Cell Function	Dialynas, D.P. et al. (1983) J. Immunol. 131:2445; Dialynas, D.P. et al. (1983) Immunol. Rev. 74:29; Kruman, I.I. et al. (1996) Cell. Immunol. 173:236; Grakoui, A. et al. (1999) Science 285:221.

Immunohistochemistry

CD4 in Mouse Intestine. CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # MAB554) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
Background	CD4
background_content	Background: CD4 CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

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Citations

1. Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity
   Authors: KR Wiehagen, NM Girgis, DH Yamada, AA Smith, SR Chan, IS Grewal, M Quigley, RI Verona
   Cancer Immunol Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model
   Authors: R Tanaka, F Goshima, S Esaki, Y Sato, T Murata, Y Nishiyama, D Watanabe, H Kimura
   Am J Cancer Res, 2017;7(8):1693-1703.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Cell depletion


3. PAX7 expression defines germline stem cells in the adult testis.
   Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
   J Clin Invest, 2014;124(9):3929-44.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


4. Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.
   Authors: Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
   Int J Cancer, 2014;134(12):2865-77.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
   J Neuroinflammation, 2012;9(0):193.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
   Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
   J. Virol., 2007;81(3):1241-50.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


7. Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.
   Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
   J. Invest. Dermatol., 2006;126(8):1792-803.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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