抗Mouse VCAM-1/CD106抗体(Anti-Mouse VCAM-1/CD106 antibody)
掲載日情報：2021/01/28 現在Webページ番号：30031
Mouse VCAM-1/CD106に対する抗体(Anti-Mouse VCAM-1/CD106 )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 08時35分現在]
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Anti-Mouse VCAM-1/CD106 MAb (Clone 112734) |
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Anti-Mouse VCAM-1/CD106 MAb (Clone 112734) |
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Anti-Mouse VCAM-1/CD106 MAb (Clone 112734) |
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[在庫・価格 ：2025年04月26日 08時35分現在]
Anti-Mouse VCAM-1/CD106 MAb (Clone 112734)
文献数： 1
- 商品コード：MAB6432-100
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：CD106 クローン：112734 Genbank No： 7412 Protein Accession No： P29533 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse VCAM-1/CD106 MAb (Clone 112734)
文献数： 1
- 商品コード：MAB6432-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse VCAM-1/CD106 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAM6433，スタンダードとして#643-VM-050を用いる。 別名：CD106 クローン：112734 Genbank No： 7412 Protein Accession No： P29533 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体 抗間葉系幹細胞マーカー抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse VCAM-1/CD106 MAb (Clone 112734)
文献数： 1
- 商品コード：MAB6432-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD106 クローン：112734 Genbank No： 7412 Protein Accession No： P29533 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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追加しました。
Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1Phe25-Glu698 (predicted)Accession # P29533 |
| Source | Monoclonal Rat IgG2A Clone # 112734 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects recombinant mouse (rm) VCAM-1 in Western blots and ELISAs. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human VCAM-1, rmICAM-1, rmE-Selectin, rmL-Selectin or rmP-Selectin. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Simple Western | 100 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Mouse VCAM-1/CD106 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse VCAM‑1/CD106 Antibody (Catalog #MAB6432 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse VCAM‑1/CD106 Biotinylated Antibody (Catalog #BAM6433 ) |
| ELISA Standard | Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF (Catalog #643-VM ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot VCAM-1/CD106 Antibody (112734) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/VCAM1_MAB6432_Western_Blot_19930.jpg) click image to view larger | Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VCAM‑1/ CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry |
![Flow Cytometry VCAM-1/CD106 Antibody (112734) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/VCAM1_MAB6432_Flow_Cytometry_12975.jpg) click image to view larger | Detection of VCAM‑1/CD106 in bEnd.3 Mouse Cell Line by Flow Cytometry.bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). |
| Simple Western |
![Simple Western VCAM-1/CD106 Antibody (112734) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/VCAM1_MAB6432_Simple_Western_20037.jpg) click image to view larger | Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 118 kDa (as indicated) using 100 µg/mL of Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VCAM-1/CD106 | VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. |
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| Long Name: | Vascular Cell Adhesion Molecule 1 | |
| Entrez Gene IDs: | 7412 (Human); 22329 (Mouse) | |
| Alternate Names: | CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 | |
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Related Research Areas
| Western Blot | |
|---|---|
 | Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VCAM‑1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry | |
 | Detection of VCAM‑1/CD106 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). |
| Simple Western | |
 | Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 118 kDa (as indicated) using 100 µg/mL of Rat Anti-Mouse VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
追加しました。
Related Product & Information
| Background | VCAM-1/CD106 |
|---|---|
| background_content | Background: VCAM-1/CD106 VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. |
追加しました。
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