抗B220抗体(Anti-B220, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Abelson mouse leukemia virus-induced pre-B tumor cells
Source	Monoclonal Rat IgG2A Clone # RA3-6B2
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse B220/CD45R.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunoprecipitation	Coffman, R.L. (1982) Immuno. Rev. 69:5.
CyTOF-reported	Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry
	B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju­gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Flow Cytometry
	Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

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Related Product & Information

Background	B220/CD45R
background_content	Background: B220/CD45R B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

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Citations

1. Combined immunotherapy with anti-PDL-1/PD-1 and anti-CD4 antibodies cures syngeneic disseminated neuroblastoma
   Authors: V Rigo, L Emionite, A Daga, S Astigiano, MV Corrias, C Quintarell, F Locatelli, S Ferrini, M Croce
   Sci Rep, 2017;7(1):14049.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
   Sci Rep, 2016;6(0):31874.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. NOTCH1 mediates a switch between two distinct secretomes during senescence
   Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
   Nat. Cell Biol, 2016;18(9):979-92.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - paraffin


4. B-cell-derived IL-10 does not vitally contribute to the clinical course of glomerulonephritis.
   Authors: Kluger M, Ostmann A, Luig M, Meyer M, Goerke B, Paust H, Meyer-Schwesinger C, Stahl R, Panzer U, Tiegs G, Steinmetz O
   Eur J Immunol, 2014;44(3):683-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Small changes in lymphocyte development and activation in mice through tissue-specific alteration of heparan sulphate.
   Authors: Garner OB, Yamaguchi Y, Esko JD, Videm V
   Immunology, 2008;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


6. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
   Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
   Virology, 2008;382(1):20-7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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