抗Human IL-17 RA/IL-17 R抗体(Anti-Human IL-17 RA/IL-17 R antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 RLeu33-Trp320Accession # Q96F46
Source	Monoclonal Mouse IgG1 Clone # 133617
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)
Flow Cytometry	2.5 µg/106 cells	K562 human chronic myelogenous leukemia cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.

Neutralization

CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17/IL‑17A induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha secretion elicited by Recombinant Human IL‑17/IL‑17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB177). The ND50 is typically 0.4-2 μg/mL.

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Related Product & Information

Long Name	Interleukin 17 Receptor
Entrez Gene IDs	23765 (Human); 16172 (Mouse); 312679 (Rat)
Background	IL-17 RA/IL-17 R
background_content	Background: IL-17 RA/IL-17 R Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

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Citations

1. Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology
   Authors: PA Szabo, A Goswami, DM Mazzuca, K Kim, DB O'Gorman, DA Hess, ID Welch, HA Young, B Singh, JK McCormick, SM Haeryfar
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway
   Authors: J Wu, J Guo, Q Cao, Y Wang, J Chen, Z Wang, Z Yuan
   Oncol Lett, 2017;13(2):770-776.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
   Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
   Eur J Immunol, 2015;46(2):440-5.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
   Authors: Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
   Proc. Natl. Acad. Sci. U.S.A., 2011;108(4):1579-84.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
   Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
   Nat. Immunol., 2011;12(12):1159-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. TH17 cytokines induce human airway smooth muscle cell migration.
   Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
   J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
   Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
   Int. J. Cancer, 2009;125(5):1092-101.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. IL-17 induces monocyte migration in rheumatoid arthritis.
   Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
   J. Immunol., 2009;182(6):3884-91.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
   Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
   Circulation, 2009;119(10):1424-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
    Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
    J. Immunol., 2008;180(1):655-63.
    Species: Human
    Sample Type: RNA
    Application: WB

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