抗EpCAM抗体(Anti-EpCAM, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：29916
EpCAMに対する抗体(Anti-EpCAM, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 00時00分現在]
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[在庫・価格 ：2024年05月18日 00時00分現在]
Anti-EpCAM, Human, Goat-Poly, Biotin
文献数： 17
- 商品コード：BAF960
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human EpCAM/TROP-1 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB9601-500，スタンダードとして#960-EP-050を用いる。 別名：17-1A Genbank No： 4072 Protein Accession No： CAA32870 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,FCM,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human EpCAM, extracelllular domainGln24-Lys265Accession # CAA32870 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human EpCAM/TROP-1 in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, recombinant mouse MAdCAM-1, rhNCAM-1, and rhNCAM-L1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human EpCAM/TROP‑1 Fc Chimera (Catalog # 960-EP) |
| Flow Cytometry | 2.5 µg/106 cells | HT‑29 human colon adenocarcinoma cell line |
| Human EpCAM/TROP-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human EpCAM/TROP‑1 Antibody (Catalog #MAB9601 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human EpCAM/TROP‑1 Biotinylated Antibody (Catalog #BAF960 ) |
| ELISA Standard | Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog #960-EP ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: EpCAM/TROP1 | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5). |
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References
| Balzar, M. et al. (1999) J. Mol. Med. 77:699. | |
| Boer, C.J, et al. (1999) J. Pathol. 188:201. | |
| Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437. | |
| Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570. | |
| Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388. | |
| Long Name: | Epithelial Cell Adhesion Molecule |
| Entrez Gene IDs: | 4072 (Human); 17075 (Mouse) |
| Alternate Names: | 17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A |
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Related Research Areas
| Adhesion Molecules in Angiogenesis | ||
| Cancer Biomarkers | ||
| Cancer Stem Cell Markers | ||
| Conventional/Classical Dendritic Cells | ||
| Embryonic and Induced Pluripotent Stem Cell Markers | ||
| Epithelial Cell Markers and Intracellular Molecules | ||
| Immunoglobulin Superfamily CAMs | ||
| Langerhans and Dermal Dendritic Cells | ||
| Surface Ectodermal Cells | ||
| Trophoblast Stem Cell Markers | ||
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Related Product & Information
| Background | EpCAM/TROP1 |
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| background_content | Background: EpCAM/TROP1 Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5). |
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Citations
- Tumor-Derived Exosomal Long Noncoding RNAs as Promising Diagnostic Biomarkers for Prostate Cancer
Authors: YH Wang, J Ji, BC Wang, H Chen, ZH Yang, K Wang, CL Luo, WW Zhang, FB Wang, XL Zhang
Cell. Physiol. Biochem., 2018;46(2):532-545.
Species: Human
Sample Type: Whole Cells
Application: Functional Assay - Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
Authors: H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho
Theranostics, 2018;8(2):505-517.
Application: Functional Assay - Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip
Authors: MH Park, E Reátegui, W Li, SN Tessier, KH Wong, AE Jensen, V Thapar, D Ting, M Toner, SL Stott, PT Hammond
J. Am. Chem. Soc., 2017;139(7):2741-2749.
Species: Human
Sample Type: Whole Cells
Application: Microfluidic Chip - Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial mesenchymal transition of gastric cancer
Aging (Albany NY), 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: ELISA detection - Circulating tumour cells as a biomarker for diagnosis and staging in pancreatic cancer
Authors: J S Ankeny
Br J Cancer, 2016;114(12):1367-75.
Species: Human
Sample Type: Whole Cells
Application: NanoVelcro Chip - Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
BMC Cancer, 2015;15(0):372.
Species: Human
Sample Type: BALF
Application: ELISA detection - Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential.
Authors: Bryan, R T, Shimwell, N J, Wei, W, Devall, A J, Pirrie, S J, James, N D, Zeegers, M P, Cheng, K K, Martin, A, Ward, D G
Br J Cancer, 2014;110(3):679-85.
Species: Human
Sample Type: Urine
Application: IP - Molecular biomarker analyses using circulating tumor cells.
Authors: Punnoose EA, Atwal SK, Spoerke JM
PLoS ONE, 2010;5(9):e12517.
Species: Human
Sample Type: Whole Cells
Application: Flow - Circulating tumor cells are transcriptionally similar to the primary tumor in a murine prostate model.
Authors: Helzer KT, Barnes HE, Day L, Harvey J, Billings PR, Forsyth A
Cancer Res., 2009;69(19):7860-6.
Species: Human
Sample Type: Whole Cells
Application: IP - High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
Authors: Burger PE, Gupta R, Xiong X, Ontiveros CS, Salm SN, Moscatelli D, Wilson EL
Stem Cells, 2009;27(9):2220-8.
Species: Mouse
Sample Type: Whole Cells
Application: Flow
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