抗VCAM-1抗体(Anti-VCAM-1, Human, Sheep-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：29888
VCAM-1に対する抗体(Anti-VCAM-1, Human, Sheep-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月21日 15時35分現在]
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Anti-VCAM-1, Human, Sheep-Poly, Biotin <Anti-CD106> |
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[在庫・価格 ：2024年05月21日 15時35分現在]
Anti-VCAM-1, Human, Sheep-Poly, Biotin <Anti-CD106>
文献数： 1
- 商品コード：BAF809
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human VCAM-1/CD106 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB809-500，スタンダードとして#809-VR-050を用いる。 別名：CD106 Genbank No： 7412 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Sheep |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human VCAM-1Extracellular domain |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human VCAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rmVCAM-1, rhICAM-1, rhICAM-2, rhICAM-3, and rhMCAM is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) |
| Human VCAM-1/CD106 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human VCAM‑1/CD106 Antibody (Catalog #MAB809 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human VCAM‑1/CD106 Biotinylated Antibody (Catalog #BAF809 ) |
| ELISA Standard | Recombinant Human VCAM-1/CD106 Protein, CF (Catalog #809-VR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VCAM-1/CD106 | Human VCAM-1 (CD106), a member of the Immunoglobulin Superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. |
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| Long Name: | Vascular Cell Adhesion Molecule 1 | |
| Entrez Gene IDs: | 7412 (Human); 22329 (Mouse) | |
| Alternate Names: | CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 | |
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Related Research Areas
| Acute Myeloid Leukemia | ||
| Adhesion Molecules in Angiogenesis | ||
| Adhesion Molecules on Endothelial Cells | ||
| Adhesion Molecules on VSMC | ||
| Adhesive Interactions in Allergy and Asthma | ||
| Adipose-derived Stem Cell Markers | ||
| Cancer Biomarkers | ||
| Endothelial Cell Differentiation Markers | ||
| Endothelial Cell Markers | ||
| Endothelial Progenitor and Endothelial Cell Markers | ||
| Hematopoietic Stem Cell Markers | ||
| Immunoglobulin Superfamily CAMs | ||
| Mesenchymal Stem Cell Markers | ||
| Mouse Tissue-Resident Macrophage Markers | ||
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Related Product & Information
| Background | VCAM-1/CD106 |
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| background_content | Background: VCAM-1/CD106 Human VCAM-1 (CD106), a member of the Immunoglobulin Superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions. |
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Citations
- IKKalpha and IKKbeta function in TNFalpha-stimulated adhesion molecule expression in human aortic smooth muscle cells.
Authors: MacKenzie CJ, Ritchie E, Paul A, Plevin R
Cell. Signal., 2007;19(1):75-80.
Species: Human
Sample Type: Cell Lysates
Application: WB
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