抗ICAM-1抗体(Anti-ICAM-1, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：29864
ICAM-1に対する抗体(Anti-ICAM-1, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2024年05月18日 16時55分現在]
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[在庫・価格 ：2024年05月18日 16時55分現在]
Anti-ICAM-1, Rat, Goat-Poly, Biotin
文献数： 0
- 商品コード：BAF583
- メーカー：RSD
- 包装：50μg
- 価格：¥113,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Rat ICAM-1/CD54 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB5832-500，スタンダードとして#583-IC-050を用いる。 別名：BB2 Genbank No： 3383 Protein Accession No： Q00238 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
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| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant rat ICAM‑1/CD54Gln28-Thr493Accession # Q00238 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat ICAM‑1/CD54 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) ICAM-1, recombinant human (rh) ICAM-1, rhICAM-3, rmICAM-5, and rmICAM-2 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.2 µg/mL | See below |
| Rat ICAM-1/CD54 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat ICAM‑1/CD54 Antibody (Catalog #MAB5832 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat ICAM‑1/CD54 Biotinylated Antibody (Catalog #BAF583 ) |
| ELISA Standard | Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF (Catalog #583-IC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot ICAM-1/CD54 Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/ICAM-1_BAF583_Western_Blot_11369.jpg) click image to view larger | Detection of Rat ICAM‑1/CD54 by Western Blot.Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF583) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: ICAM-1/CD54 | Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation. |
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References
| Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press. | |
| Siu, G. et al. (1989) J. Immunol. 143:3813. | |
| Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853. | |
| Long Name: | Intercellular Adhesion Molecule 1 |
| Entrez Gene IDs: | 3383 (Human); 15894 (Mouse); 25464 (Rat) |
| Alternate Names: | BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 |
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Related Research Areas
| Western Blot | |
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 | Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF583) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Background | ICAM-1/CD54 |
|---|---|
| background_content | Background: ICAM-1/CD54 Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation. |
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