抗VCAM-1抗体(Anti-VCAM-1, Mouse, Rat-Mono, Biotin antibody)

掲載日情報:2021/01/28 現在Webページ番号:29849

VCAM-1に対する抗体(Anti-VCAM-1, Mouse, Rat-Mono, Biotin )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-VCAM-1, Mouse, Rat-Mono(112731), Biotin <Anti-CD106>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
マッチドペア:Mouse VCAM-1/CD106 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB6432-500,スタンダードとして#643-VM-050を用いる。
別名:CD106
クローン:112731
Genbank No: 7412
Protein Accession No: P29533
法規制等
保存条件 法規備考
抗原種 Mouse 免疫動物 Rat クラス IgG 標識 Biotin
交差性 Mouse 適用 ELISA,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-VCAM-1, Mouse, Rat-Mono(112731), Biotin <Anti-CD106>

文献数: 0

説明文 マッチドペア:Mouse VCAM-1/CD106 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB6432-500,スタンダードとして#643-VM-050を用いる。
別名:CD106
クローン:112731
Genbank No: 7412
Protein Accession No: P29533
法規制等
保存条件 法規備考
抗原種 Mouse 免疫動物 Rat
交差性 Mouse 適用 ELISA,Western Blot
標識 Biotin 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityMouse
LabelBiotin
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse VCAM-1Phe25-Glu698Accession # P29533
SourceMonoclonal Rat IgG2A Clone # 112731
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse VCAM-1/CD106 in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human VCAM‑1, rmICAM-1, rmE-Selectin, rmL-Selectin, or rmP-Selectin.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse VCAM‑1/CD106 Fc Chimera (Catalog # 643-VM)
Mouse VCAM-1/CD106 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Mouse VCAM‑1/CD106 Antibody (Catalog #MAB6432 )
ELISA Detection (Matched Antibody Pair)0.5-2.0 µg/mL Mouse VCAM‑1/CD106 Biotinylated Antibody (Catalog #BAM6433 )
ELISA Standard  Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF (Catalog #643-VM )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.Preparation and Storage
ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name:Vascular Cell Adhesion Molecule 1
Entrez Gene IDs:7412 (Human); 22329 (Mouse)
Alternate Names:CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Related Research Areas

Acute Myeloid Leukemia
Adhesion Molecules in Angiogenesis
Adhesion Molecules on Endothelial Cells
Adhesion Molecules on VSMC
Adhesive Interactions in Allergy and Asthma
Adipose-derived Stem Cell Markers
Cancer Biomarkers
Endothelial Cell Differentiation Markers
Endothelial Cell Markers
Endothelial Progenitor and Endothelial Cell Markers
Hematopoietic Stem Cell Markers
Immunoglobulin Superfamily CAMs
Mesenchymal Stem Cell Markers
Mouse Tissue-Resident Macrophage Markers


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Related Product & Information

BackgroundVCAM-1/CD106
background_contentBackground:
VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.


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