抗CD11b抗体(Anti-CD11b, Rat-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Con A-activated C57BL/10 splenocytes
Source	Monoclonal Rat IgG2B Clone # M1/70
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	Mouse splenocytes
Immunohistochemistry	8-25 µg/mL
Immunoprecipitation	Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

CD11b/Integrin alpha M in Mouse Spleno­cytes. CD11b/Integrin alpha M was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin alpha M Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

CD11b/Integrin alpha M

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Background: CD11b/Integrin alpha M The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

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Citations

1. Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
   Authors: Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
   J. Virol., 2009;83(2):522-39.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


2. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
   Authors: Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
   J. Immunol., 2009;182(10):6379-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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