抗CD45抗体(Anti-CD45, Mouse, Rat-Mono, Biotin antibody)

掲載日情報:2018/11/26 現在Webページ番号:29791

CD45に対する抗体(Anti-CD45, Mouse, Rat-Mono, Biotin )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2025年06月25日 17時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-CD45, Mouse, Rat-Mono(30-F11), Biotin
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
補体依存性細胞毒性作用を有する。
別名:B220
クローン:30-F11
Genbank No: 5788
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Rat クラス IgG 標識 Biotin
交差性 Mouse 適用 FCM,IHC,IP
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事

[在庫・価格 :2025年06月25日 17時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CD45, Mouse, Rat-Mono(30-F11), Biotin

文献数: 0

説明文 補体依存性細胞毒性作用を有する。
別名:B220
クローン:30-F11
Genbank No: 5788
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Rat
交差性 Mouse 適用 FCM,IHC,IP
標識 Biotin 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事



目次に戻る

Product Details

Species ReactivityMouse
LabelBiotin
ImmunogenMouse thymus or spleen
SourceMonoclonal Rat IgG2B Clone # 30-F11
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects mouse CD45. Detects both alloantigens and all isoforms of CD45.


目次に戻る

Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry0.25 µg/106 cellsSee below
Immunohistochemistry8-25 µg/mLSee below
ImmunoprecipitationLedbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
Complement-dependent CytotoxicityLedbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.


Flow Cytometry
Detection of CD45 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # BAM114, filled histogram) or isotype control antibody (Catalog # IC013B, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
CD45 in Mouse Thymus.
CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # BAM114) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.


目次に戻る

Related Product & Information

Long NameCluster of Differentiation 45
Entrez Gene IDs5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
BackgroundCD45
background_contentBackground:
CD45
CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 30‑169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).


目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。