抗E-Selectin抗体(Anti-E-Selectin, Rat, Goat-Poly antibody)
掲載日情報:2019/10/21 現在Webページ番号:29742
E-Selectinに対する抗体(Anti-E-Selectin, Rat, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年07月17日 09時15分現在]
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Anti-E-Selectin, Rat, Goat-Poly <Anti-CD62E> |
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Anti-Rat E-Selectin/CD62E Affinity Purified Polyclonal Ab |
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[在庫・価格 :2025年07月17日 09時15分現在]
Anti-E-Selectin, Rat, Goat-Poly <Anti-CD62E>
文献数: 4
- 商品コード:AF977
- メーカー:RSD
- 包装:100μg
- 価格:¥78,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 接着阻害作用を有する。 別名:CD62 antigen-like family member E Genbank No: 6401 Protein Accession No: P98105 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Rat | 免疫動物 | Goat | ||||
交差性 | Rat | 適用 | Blocking,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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関連記事 |
Anti-Rat E-Selectin/CD62E Affinity Purified Polyclonal Ab
文献数: 1
- 商品コード:AF977-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥25,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:CD62 antigen-like family member E Genbank No: 6401 Protein Accession No: P98105 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Goat | |||||
交差性 | Rat | 適用 | Blocking,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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関連記事 |
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Product Details
Species Reactivity | Rat |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant rat E‑Selectin/CD62ETrp22-Pro494Accession # P98105 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects E‑Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse (rm) E-Selectin is observed, approximately 5% cross‑reactivity with recombinant human (rh) E-Selectin is observed and less than 1% cross‑reactivity with rmL-Selectin and rmP-Selectin is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Rat E-Selectin/CD62E Fc Chimera (Catalog # 977-ES) |
Immunohistochemistry | 5-15 µg/mL | Perfusion fixed frozen sections of rat intestine and thymus |
Adhesion Blockade | The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Rat E‑Selectin/CD62E (Catalog # 977-ES 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody. |
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Related Product & Information
Entrez Gene IDs | 6401 (Human); 20339 (Mouse); 25544 (Rat) |
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Background | E-Selectin/CD62E |
background_content | Background: E-Selectin/CD62E E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107‑115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and rat proteins share approximately 67% amino acid identity. The mouse and rat proteins share approximately 78% amino acid identity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions. |
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Citations
- Cell-based angiopoietin-1 gene therapy for acute lung injury.
Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
Am. J. Respir. Crit. Care Med., 2007;175(10):1014-26.
Species: Rat
Sample Type: Cell Lysates
Application: WB - Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
Clin. Exp. Immunol., 2007;149(2):364-71.
Species: Rat
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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