抗SPARC抗体(Anti-SPARC, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse SPARC/OsteonectinAla18-Ile302Accession # P07214
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse SPARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SPARC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Immunohistochemistry	1-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot
	Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	SPARC/Osteonectin in Mouse Embryo. SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

SPARC

background_content

Background: SPARC SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

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Citations

1. SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury
   Authors: EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai
   Front Cell Neurosci, 2018;12(0):22.
   Species: Mosue
   Sample Type: Whole Tissue
   Application: IHC-P


2. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain
   Authors: L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang
   J. Neurosci., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
   Oncotarget, 2016;7(21):31097-110.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth.
   Authors: Mathsyaraja H, Thies K, Taffany D, Deighan C, Liu T, Yu L, Fernandez S, Shapiro C, Otero J, Timmers C, Lustberg M, Chalmers J, Leone G, Ostrowski M
   Oncogene, 2015;34(28):3651-61.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
   Authors: Tseng C, Kolonin M
   Stem Cells, 2015;34(1):174-90.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Stromal niche communalities underscore the contribution of the matricellular protein SPARC to B-cell development and lymphoid malignancies.
   Authors: Sangaletti, Sabina, Tripodo, Claudio, Portararo, Paola, Dugo, Matteo, Vitali, Caterina, Botti, Laura, Guarnotta, Carla, Cappetti, Barbara, Gulino, Alessand, Torselli, Ilaria, Casalini, Patrizia, Chiodoni, Claudia, Colombo, Mario P
   Oncoimmunology, 2014;3(0):e28989.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


7. Losartan slows pancreatic tumor progression and extends survival of SPARC-null mice by abrogating aberrant TGF beta activation.
   Authors: Arnold SA, Rivera LB, Carbon JG, Toombs JE, Chang CL, Bradshaw AD, Brekken RA
   PLoS ONE, 2012;7(2):e31384.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions.
   Authors: Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK
   J. Neurosci., 2011;31(11):4154-65.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction.
   Authors: Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
   J. Exp. Med., 2009;206(1):113-23.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


10. A conserved organization of transcription during embryonic stem cell differentiation and in cells with high C value.
    Authors: Faro-Trindade I, Cook PR
    Mol. Biol. Cell, 2006;17(7):2910-20.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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