抗SPARC抗体(Anti-SPARC, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human SPARC/OsteonectinAla18-Ile303Accession # P09486
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SPARC is observed, and less than 3% cross-reactivity with recombinant human SPARC L1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	MG‑63 human osteosarcoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot
	Detection of Human SPARC by Western Blot. Western blot shows lysates of MG‑63 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SPARC at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	SPARC/Osteonectin in Human Ovary. SPARC/Osteonectin was detected in immersion fixed paraffin-embedded sections of human ovary using Goat Anti-Human SPARC/Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human SPARC by Simple WesternTM. Simple Western lane view shows lysates of MG‑63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPARC at approximately 57 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF941) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Long Name	Secreted Protein Acidic and Rich in Cysteine
Entrez Gene IDs	6678 (Human); 20692 (Mouse)
Background	SPARC
background_content	Background: SPARC SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 286 amino acid (aa), 43 kDa protein contains an N-terminal acidic region that binds calcium, a follistatin domain that contains Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure modeling shows that residues implicated in cell binding, inhibition of cell spreading, and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types undergoing an endothelial to mesenchymal transistion; its expression, however, mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9-11). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (12). Mature human SPARC shows 92%, 92%, 97%, 99%, 96%, and 85% aa identity with mouse, rat, canine, bovine, porcine, and chick SPARC, respectively.

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Citations

1. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
   Oncotarget, 2016;7(21):31097-110.
   


2. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
   Authors: Riordan D, Varma S, West R, Brown P
   PLoS ONE, 2015;10(7):e0128975.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
   Authors: Aguilera K, Rivera L, Hur H, Carbon J, Toombs J, Goldstein C, Dellinger M, Castrillon D, Brekken R
   Cancer Res, 2014;74(4):1032-44.
   Species: Human
   Sample Type: Recombinant Protein
   Application: ELISA Development


4. Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.
   BMC Cancer, 2012;12(0):464.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer.
   Authors: Chen, Jie, Wang, Mei, Xi, Bo, Xue, Jian, He, Dan, Zhang, Jie, Zhao, Yueran
   PLoS ONE, 2012;7(8):e42413.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
   Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
   Exp. Cell Res., 2011;317(7):976-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
   Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
   J. Leukoc. Biol., 2007;81(3):748-56.
   Species: Human
   Sample Type: Protein
   Application: WB

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