抗Angiotensin Converting Enzyme 2抗体(Anti-Angiotensin Converting Enzyme 2, Ectodomain, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human ACE-2Gln18-Ser740Accession # Q9BYF1
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human ACE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies

Western Blot
	Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of human ovary tissue, human testis tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	ACE‑2 in Human Kidney. ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	ACE‑2 in Human Kidney. ACE‑2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human ACE‑2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

ACE-2

background_content

Background: ACE-2 ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

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Citations

1. Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
   Authors: E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do
   Mol Ther Nucleic Acids, 2017;7(0):350-365.
   Species: Human
   Sample Type: Cell Lystaes
   Application: WB


2. HTCC: Broad Range Inhibitor of Coronavirus Entry
   Authors: Aleksandra Milewska
   PLoS ONE, 2016;11(6):e0156552.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: IHC Not Specified


3. Surface vimentin is critical for the cell entry of SARS-CoV.
   Authors: Yu Y, Chien S, Chen I, Lai C, Tsay Y, Chang S, Chang M
   J Biomed Sci, 2016;23(1):14.
   Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
   Sample Type: Whole Cells
   Application: Neut


4. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
   Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
   J Virol, 2014;88(2):1293-307.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells.
   Authors: Xiao F, Zimpelmann J, Agaybi S, Gurley S, Puente L, Burns K
   PLoS ONE, 2014;9(1):e85958.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
   Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
   Virol J, 2013;10(0):359.
   Species: Human
   Sample Type: Whole Tissue
   Application: IF


7. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor.
   Authors: Ge, Xing-Yi, Li, Jia-Lu, Yang, Xing-Lou, Chmura, Aleksei, Zhu, Guangjia, Epstein, Jonathan, Mazet, Jonna K, Hu, Ben, Zhang, Wei, Peng, Cheng, Zhang, Yu-Ji, Luo, Chu-Ming, Tan, Bing, Wang, Ning, Zhu, Yan, Crameri, Gary, Zhang, Shu-Yi, Wang, Lin-Fa, Daszak, Peter, Shi, Zheng-Li
   Nature, 2013;503(7477):535-8.
   Species: Bat
   Sample Type: Whole Cells
   Application: IF


8. Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts.
   Authors: Bertram S, Heurich A, Lavender H, Gierer S, Danisch S, Perin P, Lucas JM, Nelson PS, Pohlmann S, Soilleux EJ
   PLoS ONE, 2012;7(4):e35876.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.
   Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
   J. Biol. Chem., 2010;285(53):41935-46.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


10. Lipid rafts are involved in SARS-CoV entry into Vero E6 cells.
    Authors: Lu Y, Liu DX, Tam JP
    Biochem. Biophys. Res. Commun., 2008;369(2):344-9.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Type: Whole Cells
    Application: Flow

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