抗Gas 6抗体(Anti-Gas 6, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Gas6Asp118-Ala678Accession # NP_000811
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Gas6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Gas6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Gas6 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and human intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Gas6 at approximately 64 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Gas6 in Human Stomach Cancer Tissue. Gas6 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

Gas6

background_content

Background: Gas6 Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

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Citations

1. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
   Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
   Nat Commun, 2018;9(1):433.
   Application: Binding


2. Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
   Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
   Sci Rep, 2017;7(1):10613.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Sympathetic signaling reactivates quiescent�disseminated prostate cancer cells in the bone marrow
   Authors: A Decker, Y Jung, FC Cackowski, K Yumoto, J Wang, RS Taichman
   Mol. Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. AXL-GAS6 expression can predict for adverse prognosis in non-small cell lung cancer with brain metastases
   Authors: X Wu, W Ma, Q Zhou, H Yan, ZF Lim, M Huang, C Deng, X Yu, H Su, S Komo, H Yang, X Zhang, S Wen, Z Zhang, PC Ma
   J. Cancer Res. Clin. Oncol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids?
   Cardiovasc Res, 2013;97(1):125-33.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


6. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
   Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
   J Immunol, 2012;189(7):3508-20.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
   J. Exp. Med., 2012;209(11):2033-47.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
   Authors: Hurtado B, Munoz X, Recarte-Pelz P, Garcia N, Luque A, Krupinski J, Sala N, Garcia de Frutos P
   Thromb. Haemost., 2011;105(5):873-82.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


9. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
   Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
   Thromb. Haemost., 2011;107(1):140-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010;33(8):1840-4.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

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