抗ICAM-1抗体(Anti-ICAM-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54Gln28-Asn485Accession # Q3U8M7
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)
Immunohistochemistry	5-15 µg/mL	See below
Adhesion Blockade	The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Mouse ICAM-1 Fc Chimera (Catalog # 796-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 5 µg/mL of the antibody.

Immunohistochemistry

ICAM‑1/CD54 in Mouse Testis. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Intercellular Adhesion Molecule 1
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
Background	ICAM-1/CD54
background_content	Background: ICAM-1/CD54 Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

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Citations

1. Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance and Neurovascular Adhesion Molecule Expression
   Authors: A Niraula, Y Wang, JP Godbout, JF Sheridan
   J. Neurosci., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


2. Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
   Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
   J Am Heart Assoc, 2017;6(12):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Interferon-? treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sj�gren's Syndrome
   Authors: Z Meng, W Klinngam, MC Edman, SF Hamm-Alvar
   PLoS ONE, 2017;12(9):e0184781.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


4. Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
   Authors: CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao
   Sci Rep, 2017;7(1):717.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation
   Authors: KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis
   Sci Rep, 2017;7(1):365.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Epidermal RAF prevents allergic skin disease
   Elife, 2016;5(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


7. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
   Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
   PLoS ONE, 2015;10(3):e0119661.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.
   Authors: Goh Q, Dearth C, Corbett J, Pierre P, Chadee D, Pizza F
   Exp Cell Res, 2015;331(2):292-308.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Fresh


9. Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
   Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
   J Immunol, 2015;194(11):5061-8.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


10. Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia.
    Authors: Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V
    PLoS ONE, 2015;10(3):e0120728.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

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