抗P-Selectin抗体(Anti-P-Selectin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62PTrp42-Ala709Accession # Q01102
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Mouse and Rat P‑Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P‑Selectin/CD62P at approximately 140‑150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	P‑Selectin/CD62P in Mouse Liver. P‑Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Simple Western
	Detection of Rat P‑Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

目次に戻る

Related Product & Information

Entrez Gene IDs	6403 (Human); 20344 (Mouse)
Background	P-Selectin/CD62P
background_content	Background: P-Selectin/CD62P Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

目次に戻る

Citations

1. Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma
   Authors: A Mizrachi, Y Shamay, J Shah, S Brook, J Soong, VK Rajasekhar, JL Humm, JH Healey, SN Powell, J Baselga, DA Heller, A Haimovitz-, M Scaltriti
   Nat Commun, 2017;8(0):14292.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin
   Authors: AP Fournier, A Quenault, S Martinez d, M Gauberti, G Defer, D Vivien, F Docagne, R Macrez
   Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack
   Authors: Aurélien Quenault
   Brain, 2017;140(0):146-157.
   Application: Functional Assay


4. Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
   Authors: Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ
   Atherosclerosis, 2008;199(1):55-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
   Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
   Atherosclerosis, 2008;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets.
   Authors: Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, McVicar DW
   Blood, 2004;104(4):1042-7.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

目次に戻る