抗PD-L2抗体(Anti-PD-L2, Human, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：29669
PD-L2に対する抗体(Anti-PD-L2, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-PD-L2, Human, Goat-Poly |
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Anti-Human PD-L2/B7-DC Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-PD-L2, Human, Goat-Poly
文献数： 4
- 商品コード：AF1224
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：B7-DC Genbank No： 80380 Protein Accession No： Q9BQ51 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | Blocking,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | |||||||
Anti-Human PD-L2/B7-DC Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1224-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：B7-DC Genbank No： 80380 Protein Accession No： Q9BQ51 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | Blocking,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human PD-L2/B7-DCLeu20-Pro219Accession # Q9BQ51 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PD-L2 is observed and less than 1% cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b and recombinant rat B7-1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Immunohistochemistry | 5-15 µg/mL | Immersion fixed paraffin-embedded sections of human lung |
| Blockade of Receptor-ligand Interaction | In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) to immobilized Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL) coated at 1 µg/mL (100 µL/well). At 30 µg/mL, this antibody will block >90% of the binding. | |
| Western Blot | |
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 | Detection of Human PD‑L2/B7‑DC by Western Blot. Western blot shows lysates of human lung tissue and HDLM‑2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD‑L2/B7‑DC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1224) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD‑L2/B7‑DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Long Name | Programmed Death-1 Ligand-2 |
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| Entrez Gene IDs | 80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey) |
| Background | PD-L2/B7-DC |
| background_content | Background: PD-L2/B7-DC T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (B7-DC) (1). B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned human PD-L2 cDNA encodes a 273 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 201 aa extracellular region containing one V-like and one C-like Ig domain, a 24 aa transmembrane region, and a 28 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 70% aa sequence identity (2). PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. The other identified ligand is PD-L1. Human PD-L1 and PD-L2 share approximately 41% aa sequence identity and have similar functions. PD-L2 is broadly expressed in tissues. Highest expression was detected by Northern blot analysis in heart, placenta, liver, pancreas, spleen, and lymph node. Lower amounts of expression were observed in lung, smooth muscle, and thymus. Expression of PD-L2 on antigen presenting cell has been examined in detail. Resting B cells, monocytes and dendritic cells do not express PD-L2, expression however can be induced by LPS or BCR activation in B cells, INF-gamma treatment in monocytes, or LPS plus IFN-gamma treatment of dendritic cells. PD-L2 expression is also up regulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co‑stimulatory function for the PD-L2 on resting T cells activated with sub-optimal TCR signals has also been reported (3). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome
Authors: Joseph M Obeid
Oncoimmunology, 2016;5(11):e1235107.
Species: Human
Sample Type: Whole Tissue
Application: IHC-P - Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression
Stem Cells, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: IP - 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
Authors: Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
Int. J. Oncol., 2007;31(6):1439-47.
Species: Human
Sample Type: Whole Cells
Application: Flow
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