抗RAGE抗体(Anti-RAGE, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse RAGEGln24-Ala342Accession # O35444
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat RAGE in Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant canine RAGE is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1-1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 15-35 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE-BSA to immobilized Recombinant Mouse RAGE Fc Chimera (Catalog # 1179-RG) coated at 5 µg/mL (100 µL/well). At 166 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RAGE at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of Mouse and Rat RAGE by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	RAGE in Mouse Brain. RAGE was detected in immersion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1179) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Receptor for Advanced Glycation End Products
Entrez Gene IDs	177 (Human); 11596 (Mouse); 81722 (Rat)
Background	RAGE
background_content	Background: RAGE Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

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Citations

1. Receptor for advanced glycation endproducts (RAGE) maintains pulmonary structure and regulates the response to cigarette smoke
   Authors: L Wolf, C Herr, J Niederstra, C Beisswenge, R Bals
   PLoS ONE, 2017;12(7):e0180092.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Deletion of receptor for advanced glycation end products exacerbates lymphoproliferative syndrome and lupus nephritis in B6-MRL Fas lpr/j mice.
   Authors: Goury A, Meghraoui-Kheddar A, Belmokhtar K, Vuiblet V, Ortillon J, Jaisson S, Devy J, Le Naour R, Tabary T, Cohen J, Schmidt A, Rieu P, Toure F
   J Immunol, 2015;194(8):3612-22.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
   Authors: Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
   Immunity, 2013;38(3):541-54.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism.
   Authors: Mallidis C, Czerwiec A, Filippi S, O'Neill J, Maggi M, McClure N
   Reproduction, 2011;142(1):63-71.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
   Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
   Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


6. Type I epithelial cells are the main target of whole-body hypoxic preconditioning in the lung.
   Authors: Zhang SX, Miller JJ, Stolz DB, Serpero LD, Zhao W, Gozal D, Wang Y
   Am. J. Respir. Cell Mol. Biol., 2009;40(3):332-9.
   Species: Mouse
   Sample Type: BALF
   Application: WB


7. Neural-activity-dependent release of S100B from astrocytes enhances kainate-induced gamma oscillations in vivo.
   Authors: Sakatani S, Seto-Ohshima A, Shinohara Y, Yamamoto Y, Yamamoto H, Itohara S, Hirase H
   J. Neurosci., 2008;28(43):10928-36.
   Species: Rat
   Sample Type: In Vivo
   Application: Electrophysiology


8. Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
   Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
   J. Clin. Invest., 2008;118(1):183-94.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine.
   Authors: Mitola S, Belleri M, Urbinati C, Coltrini D, Sparatore B, Pedrazzi M, Melloni E, Presta M
   J. Immunol., 2006;176(1):12-5.
   Species: Chicken
   Sample Type: In Ovo
   Application: Neut

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