抗B7-H1抗体(Anti-B7-H1, Human, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:29648

B7-H1に対する抗体(Anti-B7-H1, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年03月29日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-B7-H1, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 12
説明文
別名:B7-H
Genbank No: 29126
Protein Accession No: Q9NZQ7
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IHC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ ニュース2018年7月1日号 p.6
ニュース2019年8月15日号 p.6

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
R&D SystemsのB7-H1/PD-L1関連製品
関連記事
Anti-Human B7-H1 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:B7-H
Genbank No: 29126
Protein Accession No: Q9NZQ7
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IHC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事

[在庫・価格 :2024年03月29日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-B7-H1, Human, Goat-Poly

文献数: 12

説明文 別名:B7-H
Genbank No: 29126
Protein Accession No: Q9NZQ7
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,IHC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ ニュース2018年7月1日号 p.6
ニュース2019年8月15日号 p.6

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
R&D SystemsのB7-H1/PD-L1関連製品
関連記事

Anti-Human B7-H1 Affinity Purified Polyclonal Ab

文献数: 1

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:B7-H
Genbank No: 29126
Protein Accession No: Q9NZQ7
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ELISA,IHC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1Phe19-Thr239Accession # Q9NZQ7
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human PD-L1/B7-H1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse PD-L1/B7-H1 is observed and less than 1% cross‑reactivity with recombinant human PD-L2 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot2 µg/mLSee below
Immunohistochemistry5-15 µg/mLSee below
Blockade of Receptor-ligand InteractionIn a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Human PD-L1/B7-H1 to immobilized Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) coated at 1 µg/mL (100 µL/well). At 50 µg/mL, this antibody will block >90% of the binding.


Western Blot
Detection of Human PD-L1/B7-H1 by Western Blot.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-HumanPD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PD-L1/B7-H1 in Human Colon and Colon Cancer Tissue.
PD-L1/B7-H1 was detected in immersion fixed paraffin-embedded sections of normal human colon (left panel) and human colon cancer tissue (right panel) using Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.


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Related Product & Information

Long NameB7 Homolog 1
Entrez Gene IDs29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
BackgroundPD-L1/B7-H1
background_contentBackground:
PD-L1/B7-H1
Human B7 homolog 1 (B7-H1), also called programmed cell death 1 ligand 1 (PDCD1L1) and programmed death ligand 1 (PDL1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 290 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Hepatitis C Virus Induces MDSCs-Like Monocytes through TLR2/PI3K/AKT/STAT3 Signaling
    Authors: N Zhai, H Li, H Song, Y Yang, A Cui, T Li, J Niu, IN Crispe, L Su, Z Tu
    PLoS ONE, 2017;12(1):e0170516.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

  2. Blocking the PD-1/PD-L1 pathway in glioma: a potential new treatment strategy
    Authors: S Xue, M Hu, V Iyer, J Yu
    J Hematol Oncol, 2017;10(1):81.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB

  3. Mesenchymal Stromal Cell Secretion of Programmed Death-1 Ligands Regulates T Cell Mediated Immunosuppression
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IP

  4. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014;9(12):e113396.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development

  5. 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
    Authors: Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
    Int. J. Oncol., 2007;31(6):1439-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  6. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006;177(12):8844-50.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development



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