抗Axl抗体(Anti-Axl, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human AxlGlu33-Pro440Accession # AAA61243
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 3-30 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Knockout Validated	Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable inAxl knockout A431 cell line.

Western Blot
	Detection of Human Axl by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Flow Cytometry
	Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
	Axl in Human Stomach. Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Knockout Validated
	Western Blot Shows Human Axl Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431cell line, but is not detectable in knockout A431 cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
	Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Axl

background_content

Background: Axl Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

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Citations

1. ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer
   Authors: Q Guo, T Grimmig, G Gonzalez, A Giobbie-Hu, G Berg, N Carr, BJ Wilson, P Banerjee, J Ma, JS Gold, B Nandi, Q Huang, AM Waaga-Gass, CG Lian, GF Murphy, MH Frank, M Gasser, NY Frank
   J. Biol. Chem., 2018;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Human Sertoli cells support high levels of Zika virus replication and persistence
   Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
   Sci Rep, 2018;8(1):5477.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
   Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
   Br. J. Cancer, 2017;117(12):1787-1797.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196
   Authors: NB Leighl, MS Tsao, G Liu, D Tu, C Ho, FA Shepherd, N Murray, JR Goffin, G Nicholas, S Sakashita, Z Chen, L Kim, J Powers, L Seymour, G Goss, PA Bradbury
   Oncotarget, 2017;8(41):69651-69662.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
   Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
   Sci Rep, 2017;7(1):10613.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
   Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
   J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


7. TAM Receptors Are Not Required for Zika Virus Infection in Mice
   Authors: AK Hastings, LJ Yockey, BW Jagger, J Hwang, R Uraki, HF Gaitsch, LA Parnell, B Cao, IU Mysorekar, CV Rothlin, E Fikrig, MS Diamond, A Iwasaki
   Cell Rep, 2017;19(3):558-568.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


8. AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses
   Authors: AS Richard, BS Shim, YC Kwon, R Zhang, Y Otsuka, K Schmitt, F Berri, MS Diamond, H Choe
   Proc. Natl. Acad. Sci. U.S.A, 2017;114(8):2024-2029.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
   Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
   Sci Rep, 2017;7(0):41389.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Zika virus cell tropism in the developing human brain and inhibition by azithromycin
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):14408-14413.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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