抗RAGE抗体(Anti-RAGE, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human RAGEGln24-Ala344Accession # Q15109
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human RAGE in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity recombinant canine RAGE is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	0.5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 4-12 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human RAGE Fc Chimera (Catalog # 1145-RG) coated at 5 µg/mL (100 µL/well). At 80 μg/mL, this antibody will block >90% of the binding.

Western Blot
	Detection of Human, Mouse, and Rat RAGE by Western Blot. Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
Immunohistochemistry
	RAGE in Human Alzheimer's Disease Brain. RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	RAGE in Human Lung. RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Long Name	Receptor for Advanced Glycation End Products
Entrez Gene IDs	177 (Human); 11596 (Mouse); 81722 (Rat)
Background	RAGE
background_content	Background: RAGE Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules. AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins. The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain. The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known. RAGE is highly expressed in the embryonic central nervous system. In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes. The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression. RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders.

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Citations

1. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration
   Authors: X Tang, JM Snowball, Y Xu, CL Na, TE Weaver, G Clair, JE Kyle, EM Zink, C Ansong, W Wei, M Huang, X Lin, JA Whitsett
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE
   PLoS ONE, 2016;11(9):e0153832.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis
   PLoS ONE, 2016;11(9):e0163330.
   Species: Human
   Sample Type: Recombinant Protein
   Application: ELISA Development


4. S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
   Authors: Iotzova-Weiss G, Dziunycz P, Freiberger S, Lauchli S, Hafner J, Vogl T, French L, Hofbauer G
   PLoS ONE, 2015;10(3):e0120971.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
   Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
   Cell Death Differ, 2015;22(7):1219-30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
   Authors: Winden D, Barton D, Betteridge B, Bodine J, Jones C, Rogers G, Chavarria M, Wright A, Jergensen Z, Jimenez F, Reynolds P
   Respir Res, 2014;15(0):129.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFbeta1.
   Authors: Basso D, Bozzato D, Padoan A, Moz S, Zambon C, Fogar P, Greco E, Scorzeto M, Simonato F, Navaglia F, Fassan M, Pelloso M, Dupont S, Pedrazzoli S, Fassina A, Plebani M
   Cell Commun Signal, 2014;12(0):20.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
   Authors: Sessa L, Gatti E, Zeni F, Antonelli A, Catucci A, Koch M, Pompilio G, Fritz G, Raucci A, Bianchi M
   PLoS ONE, 2014;9(1):e86903.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
   Authors: Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H
   PLoS ONE, 2014;9(4):e94118.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
    Authors: Monden, Masayo, Koyama, Hidenori, Otsuka, Yoshiko, Morioka, Tomoaki, Mori, Katsuhit, Shoji, Takuhito, Mima, Yohei, Motoyama, Koka, Fukumoto, Shinya, Shioi, Atsushi, Emoto, Masanori, Yamamoto, Yasuhiko, Yamamoto, Hiroshi, Nishizawa, Yoshiki, Kurajoh, Masafumi, Yamamoto, Tetsuya, Inaba, Masaaki
    Diabetes, 2013;62(2):478-89.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence

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