抗PDGF Receptor α抗体(Anti-PDGF Receptor α, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)Accession # P26618
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human PDGF‑AA.

Western Blot
	Detection of Mouse PDGF R alpha by Western Blot. Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	PDGF R alpha in Mouse Embryo. PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	Cell Proliferation Induced by PDGF‑AA and Neutralization by Mouse PDGF R alpha Antibody. Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF‑AA (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Long Name	Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
Background	PDGF R alpha
background_content	Background: PDGF R alpha The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

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Citations

1. B-1a lymphocytes promote oligodendrogenesis during brain development
   Authors: S Tanabe, T Yamashita
   Nat. Neurosci., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Role of P2XReceptor in Mouse Voiding Function
   Authors: W Yu, WG Hill, SC Robson, ML Zeidel
   Sci Rep, 2018;8(1):1838.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


3. Pharmacogenetic stimulation of neuronal activity increases myelination in an axon-specific manner
   Authors: S Mitew, I Gobius, LR Fenlon, SJ McDougall, D Hawkes, YL Xing, H Bujalka, AL Gundlach, LJ Richards, TJ Kilpatrick, TD Merson, B Emery
   Nat Commun, 2018;9(1):306.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
   Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
   PLoS ONE, 2017;12(12):e0189380.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


5. Pdgfr? functions in endothelial-derived cells to regulate neural crest cells and development of the great arteries
   Authors: H Aghajanian, YK Cho, NW Rizer, Q Wang, L Li, K Degenhardt, R Jain
   Dis Model Mech, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. ALS skeletal muscle shows enhanced TGF-? signaling, fibrosis and induction of fibro/adipogenic progenitor markers
   Authors: D Gonzalez, O Contreras, DL Rebolledo, JP Espinoza, B van Zunder, E Brandan
   PLoS ONE, 2017;12(5):e0177649.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
   Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
   PLoS ONE, 2017;12(8):e0182265.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Colonic PDGFR? Overexpression Accompanied Forkhead Transcription Factor FOXO3 Up-Regulation in STZ-Induced Diabetic Mice
   Authors: H Lu, C Zhang, N Song, C Lu, L Tong, X Huang, YC Kim, J Chen, W Xu
   Cell. Physiol. Biochem., 2017;43(1):158-171.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Physiologically activated mammary fibroblasts promote postpartum mammary cancer
   Authors: Q Guo, J Minnier, J Burchard, K Chiotti, P Spellman, P Schedin
   JCI Insight, 2017;2(6):e89206.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Leptin sustains spontaneous remyelination in the adult central nervous system
    Authors: K Matoba, R Muramatsu, T Yamashita
    Sci Rep, 2017;7(0):40397.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed

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