抗PD-1抗体(Anti-PD-1, Mouse, Goat-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：29581
PD-1に対する抗体(Anti-PD-1, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月03日 19時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-PD-1, Mouse, Goat-Poly |
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Anti-Mouse PD-1 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月03日 19時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-PD-1, Mouse, Goat-Poly
文献数： 9
- 商品コード：AF1021
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：CD279 Genbank No： 5133 Protein Accession No： Q02242 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Goat | ||||
| 交差性 | Mouse | 適用 | FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | |||||||
Anti-Mouse PD-1 Affinity Purified Polyclonal Ab
文献数： 4
- 商品コード：AF1021-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：CD279 Genbank No： 5133 Protein Accession No： Q02242 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Mouse | 適用 | FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-1Leu25-Gln167Accession # Q02242 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse PD-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human PD‑1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.2 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | Mouse splenocytes treated with PHA |
| Immunohistochemistry | 5-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Western Blot | |
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 | Detection of Mouse PD‑1 by Western Blot. Western blot shows lysates of EL‑4 mouse lymphoblast cell line and CTLL‑2 mouse cytotoxic T cell line (negative control). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD‑1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Mouse PD‑1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line mock transfected or transfected with mouse PD-1. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD‑1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry | |
 | PD‑1 in Mouse Thymus. PD‑1 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
| Immunohistochemistry | |
 | PD‑1 in Mouse Spleen. PD‑1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1021) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
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Related Product & Information
| Background | PD-1 |
|---|---|
| background_content | Background: PD-1 Programmed Death-1 (PD-1) is type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- CD8?? intraepithelial lymphocytes arise from two main thymic precursors
Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
Nat. Immunol., 2017;0(0):.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC - Anti-programmed cell death 1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W F1 mice.
Authors: Kasagi S, Kawano S, Okazaki T, Honjo T, Morinobu A, Hatachi S, Shimatani K, Tanaka Y, Minato N, Kumagai S
J. Immunol., 2010;184(5):2337-47.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen - Depletion of the programmed death-1 receptor completely reverses established clonal anergy in CD4(+) T lymphocytes via an interleukin-2-dependent mechanism.
Authors: Bishop KD, Harris JE, Mordes JP, Greiner DL, Rossini AA, Czech MP, Phillips NE
Cell. Immunol., 2009;256(1):86-91.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
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