抗B7-H1抗体(Anti-B7-H1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1Phe19-Thr238Accession # Q9EP73
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human PD-L1/B7-H1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	Perfusion fixed frozen sections of mouse small intestine (Peyer's patch) and thymus
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Mouse PD-L1/B7-H1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-MousePD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection ofPD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1019) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

PD-L1/B7-H1

background_content

Background: PD-L1/B7-H1 Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1‑4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7‑H1/PD‑L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1‑4).

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Citations

1. Interferon-gamma drives programmed death-ligand 1 expression on islet ? cells to limit T cell function during autoimmune diabetes
   Authors: KC Osum, AL Burrack, T Martinov, NL Sahli, JS Mitchell, CG Tucker, KE Pauken, K Papas, B Appakalai, JA Spanier, BT Fife
   Sci Rep, 2018;8(1):8295.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. PD-L1 checkpoint inhibition and anti-CTLA-4 whole tumor cell vaccination counter adaptive immune resistance: A mouse neuroblastoma model that mimics human disease
   Authors: P Srinivasan, X Wu, M Basu, C Rossi, AD Sandler
   PLoS Med., 2018;15(1):e1002497.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


3. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
   Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
   J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Tumor localized secretion of soluble PD1 enhances oncolytic virotherapy
   Authors: MY Bartee, KM Dunlap, E Bartee
   Cancer Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Hormonal vitamin D upregulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in human but not mouse
   Authors: V Dimitrov, M Bouttier, G Boukhaled, R Salehi-Tab, R Avramescu, B Memari, B Hasaj, GL Lukacs, CM Krawczyk, JH White
   J. Biol. Chem., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. PD-1/PD-L1 blockade enhances T cell activity and antitumor efficacy of imatinib in gastrointestinal stromal tumors
   Clin Cancer Res, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


7. Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas
   Int J Cancer, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


8. beta-Cell-targeted blockage of PD1 and CTLA4 pathways prevents development of autoimmune diabetes and acute allogeneic islets rejection.
   Authors: El Khatib M, Sakuma T, Tonne J, Mohamed M, Holditch S, Lu B, Kudva Y, Ikeda Y
   Gene Ther, 2015;22(5):430-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. IFN-gamma from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer.
   Authors: Abiko K, Matsumura N, Hamanishi J, Horikawa N, Murakami R, Yamaguchi K, Yoshioka Y, Baba T, Konishi I, Mandai M
   Br J Cancer, 2015;112(9):1501-9.
   


10. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2013;133(2):334-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified

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