抗Cathepsin D抗体(Anti-Cathepsin D, Human, Goat-Poly antibody)
掲載日情報：2019/10/21 現在Webページ番号：29578
Cathepsin Dに対する抗体(Anti-Cathepsin D, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年11月03日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Cathepsin D, Human, Goat-Poly |
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Anti-Human Cathepsin D Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年11月03日 13時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Cathepsin D, Human, Goat-Poly
文献数： 11
- 商品コード：AF1014
- メーカー：RSD
- 包装：100μg
- 価格：¥83,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：cathepsin D Genbank No： 1509 Protein Accession No： P07339 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | IHC,IP,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | |||||||
Anti-Human Cathepsin D Affinity Purified Polyclonal Ab
文献数： 9
- 商品コード：AF1014-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：cathepsin D Genbank No： 1509 Protein Accession No： P07339 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | IHC,IP,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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| 関連記事 | |||||||
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Cathepsin DLeu21-Leu412Accession # P07339 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) Cathepsin D and less than 5% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin C, rhCathepsin H, rhCathepsin L, and rhCathepsin Z is observed. In Western blots, approximately 20% cross-reactivity with rmCathepsin D and less than 1% cross-reactivity with rhBACE-1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Simple Western | 50 µg/mL | See below |
| Immunohistochemistry | 5-15 µg/mL | See below |
| Immunoprecipitation | 25 µg/mL | Conditioned cell culture medium spiked with Recombinant Human Cathepsin D (Catalog # 1014-AS), see our available Western blot detection antibodies |
| Western Blot | |
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 | Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of PANC‑1 human pancreatic carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017).Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry | |
 | Cathepsin D in Human Lung Cancer Tissue. Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Simple Western | |
 | Detection of Human Cathepsin D by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Procathepsin D at approximately 55 kDa and Cathepsin D heavy chain at approximately 37 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
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Related Product & Information
| Background | Cathepsin D |
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| background_content | Background: Cathepsin D Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19-64), and a mature chain (residues 65‑412) (2‑4). The mature chain can be processed further to the light (residues 65‑161) and heavy (residues 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC
Authors: J Han, LA Goldstein, W Hou, S Chatterjee, TF Burns, H Rabinowich
Autophagy, 2018;0(0):1-14.
Species: Human
Sample Type: Tissue Homogenates
Application: WB - Amphiphilic star PEG-Camptothecin conjugates for intracellular targeting
J Control Release, 2016;0(0):.
Species: Human
Sample Type: Whole Cells
Application: IHC - Fresh - The Apaf-1-binding protein Aven is cleaved by Cathepsin D to unleash its anti-apoptotic potential.
Cell Death Differ., 2012;19(9):1435-45.
Species: Human
Sample Type: Cell Lysates
Application: WB - IFN-gamma regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells.
Authors: Khalkhali-Ellis Z, Abbott DE, Bailey CM, Goossens W, Margaryan NV, Gluck SL, Reuveni M, Hendrix MJ
J. Cell. Biochem., 2008;105(1):208-18.
Species: Human
Sample Type: Cell Lysates
Application: WB - Clathrin is a key regulator of basolateral polarity.
Authors: Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E
Nature, 2008;452(7188):719-23.
Species: Canine
Sample Type: Cell Lysates
Application: IP
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