抗UNC5H2抗体(Anti-UNC5H2, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant rat UNC5H2Gly27-Asp373Accession # O08722
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat UNC5H2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) UNC5H3 and recombinant rat UNC5H1 is observed, and less than 1% cross-reactivity with rhUNC5H4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	Immersion fixed frozen sections of rat brain

Western Blot

Detection of Rat UNC5H2 by Western Blot. Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Rat UNC5H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for UNC5H2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

UNC5H2/UNC5B

background_content

Background: UNC5H2/UNC5B Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6). Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

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Citations

1. Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells
   Authors: K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto
   Neuropharmacology, 2017;119(0):26-39.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


2. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
   Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
   Nat Med, 2014;20(4):377-84.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
   Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
   EMBO J, 2013;32(5):629-44.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
   Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
   Arterioscler Thromb Vasc Biol, 2013;33(5):911-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury.
   Authors: Konig K, Kohler D, Granja T, Jennewein C, Tran N, Mirakaj V, Krohnert F, Rosenberger P
   PLoS ONE, 2012;7(7):e41085.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
   Authors: Tadagavadi RK, Wang W
   J. Immunol., 2010;185(0):3750-8.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
   Authors: Hoang S, Liauw J, Choi M
   J. Cereb. Blood Flow Metab., 2009;29(2):385-97.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
   Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
   Am. J. Pathol., 2009;175(3):1010-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Netrin-1 inhibits leukocyte migration in vitro and in vivo.
   Authors: Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB
   Proc. Natl. Acad. Sci. U.S.A., 2005;102(41):14729-34.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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