抗VE-Cadherin抗体(Anti-VE-Cadherin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VE-CadherinAsp46-Gln592Accession # 2208309A
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	3-25 µg/mL	See below

Western Blot
	Detection of Mouse VE‑Cadherin by Western Blot. Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	VE‑Cadherin in Mouse Heart. VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Simple Western
	Detection of Mouse VE‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

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Related Product & Information

Long Name	Vascular Endothelium Cadherin
Entrez Gene IDs	1003 (Human); 12562 (Mouse)
Background	VE-Cadherin
background_content	Background: VE-Cadherin The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

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Citations

1. The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation
   Authors: I Milo, R Blecher-Go, Z Barnett-It, R Bar-Ziv, O Tal, I Gurevich, T Feferman, I Drexler, I Amit, P Bousso, G Shakhar
   Eur. J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


2. CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis
   Authors: L Guo, H Akahori, E Harari, SL Smith, R Polavarapu, V Karmali, F Otsuka, RL Gannon, RE Braumann, MH Dickinson, A Gupta, AL Jenkins, MJ Lipinski, J Kim, P Chhour, PS de Vries, H Jinnouchi, R Kutys, H Mori, MD Kutyna, S Torii, A Sakamoto, CU Choi, Q Cheng, ML Grove, MA Sawan, Y Zhang, Y Cao, FD Kolodgie, DP Cormode, DE Arking, E Boerwinkle, AC Morrison, J Erdmann, N Sotoodehni, R Virmani, AV Finn
   J. Clin. Invest., 2018;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis
   Authors: J Cao, M Ehling, S März, J Seebach, K Tarbashevi, T Sixta, ME Pitulescu, AC Werner, B Flach, E Montanez, E Raz, RH Adams, H Schnittler
   Nat Commun, 2017;8(1):2210.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
   Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
   Stem Cell Reports, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming
   Authors: L Tian, A Goldstein, H Wang, H Ching Lo, I Sun Kim, T Welte, K Sheng, LE Dobrolecki, X Zhang, N Putluri, TL Phung, SA Mani, F Stossi, A Sreekumar, MA Mancini, WK Decker, C Zong, MT Lewis, XH Zhang
   Nature, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells
   Authors: Jingzhu Zhang
   J Bone Miner Res, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


7. HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver
   JCI Insight, 2016;1(21):e87058.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells
   Nat Commun, 2016;7(0):12422.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
   Authors: Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D
   Am J Pathol, 2016;186(1):87-100.
   Species: Mouse
   Sample Type: Recombinant Protein
   Application: WB


10. Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts.
    Authors: Zang G, Gustafsson K, Jamalpour M, Hong J, Genove G, Welsh M
    BMC Cancer, 2015;15(0):234.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

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