抗STAT1抗体(Anti-STAT1, p91, Goat-Poly antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human STAT1
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse STAT1 p91 in Western blots. In Western blots, less than 1% cross-reactivity with human or mouse STAT1 p84 is observed. Additionally, no cross-reactivity with the other STATs has been detected by immunoprecipitation or Western blotting.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25-1 µg/mL	See below
Immunoprecipitation	3 µg/106 cells	See below
Knockout Validated	STAT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT1 knockout HeLa cell line.

Western Blot
	Detection of Human and Mouse STAT1 p91 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunoprecipitation
	Immunoprecipitation of Human STAT1. STAT1 p91 was immunoprecipitated from 200 μg of cell lysate of HeLa human cervical epithelial carcinoma cell line following incubation with 3 µg Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) overnight at 4 °C. STAT1 p91-antibody complexes were absorbed using Protein G sepharose (Invitrogen, Catalog # 10-1242). Immunoprecipitated STAT1 p91 was detected by Western blot using 1 µg/mL Human STAT1 Monoclonal Antibody (Catalog # MAB14901). View our recommended buffer recipes for immunoprecipitation.
Knockout Validated
	Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
	Western Blot Shows Human STAT1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Signal Transducer and Activator of Transcription 1
Background	STAT1
background_content	Background: STAT1 STAT1 is a member of the STAT family of cytoplasmic transcription factors that mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1a at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).

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Citations

1. Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation
   Authors: II Nitulescu, SC Meyer, QJ Wen, JD Crispino, ME Lemieux, RL Levine, HE Pelish, MD Shair
   EBioMedicine, 2017;26(0):112-125.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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