抗Human CX3CL1/Fractalkine抗体(Anti-Human CX3CL1/Fractalkine antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0-derived and E. coli-derived Recombinant Human CX3CL1/FracktalkineGln25-Arg339Accession # P78423
Source	Monoclonal Mouse IgG1 Clone # 81506
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.

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Applications and Data

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Human CX3CL1/Fractalkine Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog #MAB3652 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog #BAM365 )
ELISA Standard		Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog #365-FR )
Neutralization	Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody.Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CX3CL1/Fractalkine	CX3CL1, also known as Fractalkine, is a type I membrane protein inwhich a chemokine domain possessing a unique C-X3-C cysteine motif istethered on a long mucin-like stalk. It can also be released as asoluble molecule upon proteolysis at a membrane proximal site.
Entrez Gene IDs:	6376 (Human); 20312 (Mouse); 89808 (Rat)
Alternate Names:	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Related Research Areas

Cancer Stem Cell Markers

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

CX3C Chemokines and Receptors

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Renal Markers

Special Glycoproteins

Uptake of Amyloid beta by Macrophages

Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Arthritis Res. Ther, 2017;19(1):45. Species: Human Sample Type: Serum Application: ELISA Capture

Neutralization

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
Background	CX3CL1/Fractalkine
background_content	Background: CX3CL1/Fractalkine CX3CL1, also known as Fractalkine, is a type I membrane protein inwhich a chemokine domain possessing a unique C-X3-C cysteine motif istethered on a long mucin-like stalk. It can also be released as asoluble molecule upon proteolysis at a membrane proximal site.

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Citations

1. Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
   Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
   Arthritis Res. Ther, 2017;19(1):45.
   Species: Human
   Sample Type: Serum
   Application: ELISA Capture


2. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
   Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
   Mediators Inflamm., 2017;2017(0):1515389.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells.
   Authors: Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H
   Kidney Int, 2015;87(6):1153-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
   Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
   Mucosal Immunol, 2012;5(0):702.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells.
   Authors: Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP
   Cancer Lett., 2009;273(1):127-39.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration.
   Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T
   J. Leukoc. Biol., 2008;84(1):215-23.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


7. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
   Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
   J. Immunol., 2007;178(12):8064-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Fractalkine mediates natural killer-dependent antitumor responses in vivo.
   Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
   Cancer Res., 2003;63(21):7468-74.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISA Development

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