抗GM-CSF抗体(Anti-GM-CSF, Mouse-Mono antibody)
掲載日情報:2019/10/21 現在Webページ番号:29524
GM-CSFに対する抗体(Anti-GM-CSF, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-GM-CSF, Mouse-Mono(83308) |
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Anti-Rat GM-CSF MAb (Clone 83308) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-GM-CSF, Mouse-Mono(83308)
文献数: 2
- 商品コード:MAB5181
- メーカー:RSD
- 包装:500μg
- 価格:¥122,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:colony stimulating factor 2 (granulocyte-macrophage) クローン:83308 Genbank No: 1437 Protein Accession No: P48750 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Rat | 免疫動物 | Mouse | ||||
交差性 | Rat | 適用 | FCM,IC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 |
Anti-Rat GM-CSF MAb (Clone 83308)
文献数: 0
- 商品コード:MAB5181-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:colony stimulating factor 2 (granulocyte-macrophage) クローン:83308 Genbank No: 1437 Protein Accession No: P48750 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Rat | 適用 | FCM,IC,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) 使いっきり抗体 |
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関連記事 |
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Product Details
Species Reactivity | Rat |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant rat GM-CSFAla1-Lys127Accession # P48750 |
Source | Monoclonal Mouse IgG2B Clone # 83308 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects rat GM-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant GM‑CSF from mouse, human, or pig is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Rat GM-CSF (Catalog # 518-GM) under non-reducing conditions only. Catalog # AF518 is recommended to detect rat GM-CSF in Western blots. |
CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
Immunocytochemistry | 8-25 µg/mL | See below |
Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | Rat splenocytes fixed with paraformaldehyde and permeabilized with saponin |
Neutralization | Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J. N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.5 ng/mL Recombinant Rat GM‑CSF. |
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Related Product & Information
Long Name | Granulocyte Macrophage Growth Factor |
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Entrez Gene IDs | 1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline) |
Background | GM-CSF |
background_content | Background: GM-CSF GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8‑10). Mature rat GM-CSF shares 56%‑69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha (13). Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells (14, 15). |
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Citations
- Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats.
Authors: Stojic-Vukanic Z, Nacka-Aleksic M, Pilipovic I, Vujnovic I, Blagojevic V, Kosec D, Dimitrijevic M, Leposavic G
Immun Ageing, 2015;12(0):16.
Species: Rat
Sample Type: Whole Cells
Application: Flow - Effect of Lactobacillus acidophilus supernatants on body weight and leptin expression in rats.
Authors: Sousa R, Halper J, Zhang J
BMC Complement Altern Med, 2008;8(0):5.
Species: Rat
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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