抗Fractalkine抗体(Anti-Fractalkine, Human, Mouse-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	NS0-derived and E.coli-derived Recombinant Human FractalkineGln25-Arg339Accession # P78423
Source	Monoclonal Mouse IgG1 Clone # 51637
Purification	Protein A or G purified from ascites
Specificity	Detects human CX3CL1 in ELISAs and Western blots.  In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)
Human CX3CL1/Fractalkine Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog #MAB3652 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog #BAM365 )
ELISA Standard		Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog #365-FR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CX3CL1/Fractalkine	Fractalkine is a type I membrane protein in which a chemokine domainpossessing a unique C-X3-C cysteine motif is tethered on a longmucin-like stalk. It can also be released as a soluble molecule uponproteolysis at a membrane proximal site.
Entrez Gene IDs:	6376 (Human); 20312 (Mouse); 89808 (Rat)
Alternate Names:	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Related Research Areas

Cancer Stem Cell Markers

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

CX3C Chemokines and Receptors

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Renal Markers

Special Glycoproteins

Uptake of Amyloid beta by Macrophages

Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Arthritis Res. Ther, 2017;19(1):45. Species: Human Sample Type: Serum Application: ELISA detection

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Related Product & Information

Background	CX3CL1/Fractalkine
background_content	Background: CX3CL1/Fractalkine Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

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Citations

1. Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
   Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
   Arthritis Res. Ther, 2017;19(1):45.
   Species: Human
   Sample Type: Serum
   Application: ELISA detection


2. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
   Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
   Mediators Inflamm., 2017;2017(0):1515389.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


3. Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration.
   Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T
   J. Leukoc. Biol., 2008;84(1):215-23.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
   Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
   J. Immunol., 2007;178(12):8064-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Fractalkine mediates natural killer-dependent antitumor responses in vivo.
   Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
   Cancer Res., 2003;63(21):7468-74.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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