抗B220抗体(Anti-B220, Rat-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	Abelson mouse leukemia virus-induced pre-B tumor cells
Source	Monoclonal Rat IgG2A Clone # RA3-6B2
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse B220/CD45R. Reacts with the extracellular domain of mouse B220/CD45R antigen.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunoprecipitation	Coffman, R.L. (1982) Immuno. Rev. 69:5.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

B220/CD45R

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Background: B220/CD45R B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

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Citations

1. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
   Alzheimers Res Ther, 2016;8(1):54.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Effects of a germ-free environment on gut immune regulation and diabetes progression in non-obese diabetic (NOD) mice.
   Authors: Alam C, Bittoun E, Bhagwat D, Valkonen S, Saari A, Jaakkola U, Eerola E, Huovinen P, Hanninen A
   Diabetologia, 2011;54(6):1398-406.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
   Authors: Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
   Arthritis Rheum., 2006;54(7):2300-5.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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