抗IL-12抗体(Anti-IL-12, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12 heterodimerArg23-Ser219 of p35; Ile23-Ser328 of p40Accession # P29459 (p35) & P29460 (p40)
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse IL-12 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑12 (Catalog # 219-IL)
Immunocytochemistry	5-15 µg/mL	Immersion fixed human peripheral blood mononuclear cells
Neutralization	Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND50) is typically 0.03-0.05 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑12.

Neutralization

Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND50 is typically 0.03‑0.05 µg/mL.

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Related Product & Information

Long Name	Interleukin 12
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
Background	IL-12
background_content	Background: IL-12 Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

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Citations

1. Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells.
   Authors: Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S
   Int. Immunol., 2008;20(10):1321-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
   Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
   Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Innate immune responses to herpes simplex virus type 2 influence skin homing molecule expression by memory CD4+ lymphocytes.
   Authors: Koelle DM, Huang J, Hensel MT, McClurkan CL
   J. Virol., 2006;80(6):2863-72.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes.
   Authors: Coward WR, Marei A, Yang A, Vasa-Nicotera MM, Chow SC
   J. Immunol., 2006;176(9):5284-92.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
   Authors: Nagarajan S, Selvaraj P
   Vaccine, 2005;24(13):2264-74.
   Species: Human
   Sample Type: Cell Lysates
   Application: Affinity Chromatography


7. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
   Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
   J. Immunol., 2005;174(4):2376-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
   Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
   J Pharm Sci, 2005;94(5):974-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
   Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
   J. Immunol., 2004;172(1):130-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. APC-independent activation of NK cells by the Toll-like receptor 3 agonist double-stranded RNA.
    Authors: Schmidt KN, Leung B, Kwong M, Zarember KA, Satyal S, Navas TA, Wang F, Godowski PJ
    J. Immunol., 2004;172(1):138-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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