抗Human NKp46/NCR1抗体(Anti-Human NKp46/NCR1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse T cell hybridoma transfected with human NKp46/NCR1
Source	Monoclonal Mouse IgG2B Clone # 195314
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human NKp46/NCR1 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
Flow Cytometry	2.5 µg/106 cells	Human whole blood CD56+ natural killer cells
Agonist Activity	Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. The ED50 for this effect is typically 0.1‑0.3 μg/mL.
CyTOF-reported	Horowitz, A. et al. Sci. Transl. Med. (2013) 208ra145. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

NKp46/NCR1 in NK‑92 Human Cell Line. NKp46/NCR1 was detected in immersion fixed NK‑92 human natural killer lymphoma cell line using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Entrez Gene IDs	9437 (Human); 17086 (Mouse)
Background	NKp46/NCR1
background_content	Background: NKp46/NCR1 NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

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Citations

1. Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma
   Authors: SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci
   Cancer Discov, 2018;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models
   Authors: Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin
   J Neuroinflammation, 2017;14(1):248.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
   Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
   Sci Rep, 2017;7(0):44544.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


4. Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome
   J Immunol, 2016;197(5):1621-30.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
   Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
   Oncoimmunology, 2016;5(12):e1154251.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


6. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
   Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
   Immunity, 2016;45(1):131-44.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.
   Authors: Messaoudene M, Perier A, Fregni G, Neves E, Zitvogel L, Cremer I, Chanal J, Sastre-Garau X, Deschamps L, Marinho E, Larousserie F, Maubec E, Avril M, Caignard A
   PLoS ONE, 2015;10(7):e0133363.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


8. A novel tetravalent bispecific TandAb (CD30/CD16A) efficiently recruits NK cells for the lysis of CD30+ tumor cells.
   Authors: Reusch U, Burkhardt C, Fucek I, Le Gall F, Le Gall M, Hoffmann K, Knackmuss S, Kiprijanov S, Little M, Zhukovsky E
   MAbs, 2014;6(3):728-39.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


9. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
   Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
   J Immunol, 2014;192(8):3805-15.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


10. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012;9(0):1.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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