抗CD83抗体(Anti-CD83, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83
Source	Monoclonal Mouse IgG1 Clone # HB15e
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CD83.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of CD83 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells were stained with Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
	CD83 in Human Dendritic Cells. CD83 was detected in immersion fixed human dendritic cells using 10 µg/mL Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
	CD83 in THP‑1 Human Cell Line. CD83 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human CD83 Monoclonal Antibody (Catalog # MAB1774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

CD83

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Background: CD83 Human CD83 is a 40‑50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

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Citations

1. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
   Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J
   PLoS Biol, 2012;10(12):e1001448.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18.
   Authors: Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F
   Arthritis Rheum., 2008;58(1):251-62.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
   Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
   J. Pathol., 2008;214(3):328-36.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


4. Interleukin-18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies.
   Authors: Tucci M, Quatraro C, Dammacco F, Silvestris F
   Clin. Exp. Immunol., 2006;146(1):21-31.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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