抗Mouse CCL20/MIP-3 alpha抗体(Anti-Mouse CCL20/MIP-3 alpha antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL20/MIP-3 alpha Ala28-Met97Accession # O89093
Source	Monoclonal Rat IgG1 Clone # 114908
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL20/MIP-3 alpha in ELISAs. Does not cross-react with recombinant human (rh) CCL19, rhCCL20, recombinant mouse (rm) CCL3, 4, 9, 19, rmCXCL2, or recombinant rat CCL20.

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Applications and Data

	Recommended Concentration	Sample
Mouse CCL20/MIP-3 alpha Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CCL20/MIP‑3 alpha Antibody (Catalog #MAB7601 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog #BAF760 )
ELISA Standard		Recombinant Mouse CCL20/MIP-3 alpha Protein (Catalog #760-M3 )
Neutralization	Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody.Recombinant Mouse CCL20/MIP‑3 alpha (Catalog # 760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601). The ND50 is typically 3-15 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha	MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively. MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

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References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Entrez Gene IDs:	6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names:	beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Related Research Areas

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Angiogenesis

Macrophage Activation Markers

Molecules Secreted by VSMC

Regulatory T Cells (Tregs)

Th17 Cells

IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie Cell. Physiol. Biochem., 2017;42(5):1961-1972. Species: Mouse Sample Type: Serum Application: ELISA Capture

Neutralization

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601). The ND50 is typically 3-15 µg/mL.

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Related Product & Information

Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
Background	CCL20/MIP-3 alpha
background_content	Background: CCL20/MIP-3 alpha MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

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Citations

1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


2. Autoimmune Renal Disease Is Exacerbated by S1P-Receptor-1-Dependent Intestinal Th17 Cell Migration to the Kidney
   Authors: Ulf Panzer
   Immunity, 2016;45(5):1078-1092.
   Species: Mouse
   Sample Type:
   Application: In vivo


3. The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
   Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
   Mol Cancer, 2015;14(0):115.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


4. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
   Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
   J Invest Dermatol, 2013;133(1):164-71.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


5. Tc17 Cells Mediate Vaccine Immunity against Lethal Fungal Pneumonia in Immune Deficient Hosts Lacking CD4+ T Cells.
   Authors: Nanjappa SG, Heninger E, Wuthrich M, Gasper DJ, Klein BS
   PLoS Pathog., 2012;8(7):e1002771.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. CCL20/CCR6 blockade enhances immunity to RSV by impairing recruitment of DC.
   Authors: Kallal LE, Schaller MA, Lindell DM, Lira SA, Lukacs NW
   Eur. J. Immunol., 2010;40(4):1042-52.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
   Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
   J. Immunol., 2009;182(5):3055-62.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. Tumor-specific Th17-polarized cells eradicate large established melanoma.
   Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
   Blood, 2008;112(2):362-73.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. The chemokine receptor CCR6 is an important component of the innate immune response.
   Authors: Wen H, Hogaboam CM, Lukacs NW, Cook DN, Lira SA, Kunkel SL
   Eur. J. Immunol., 2007;37(9):2487-98.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo

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